7FJO

Cryo-EM structure of South African (B.1.351) SARS-CoV-2 spike glycoprotein in complex with three T6 Fab

7FJO の概要

• エントリーDOI: 10.2210/pdb7fjo/pdb
• EMDBエントリー: 31625
• 分子名称: T6 heavy chain, T6 light chain, Spike glycoprotein, ... (4 entities in total)
• 機能のキーワード: sars-cov-2, spike, antibody, viral protein, immune system-viral protein complex, immune system/viral protein
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 572109.18

構造登録者

Wang, X.,Zhang, L.,Zhang, S.,Liang, Q. (登録日: 2021-08-04, 公開日: 2022-04-13, 最終更新日: 2024-11-13)

主引用文献

Liang, Q.,Wang, Y.,Zhang, S.,Sun, J.,Sun, W.,Li, J.,Liu, Y.,Li, M.,Cheng, L.,Jiang, Y.,Wang, R.,Zhang, R.,Yang, Z.,Ren, Y.,Chen, P.,Gao, P.,Yan, H.,Zhang, Z.,Zhang, Q.,Shi, X.,Wang, J.,Liu, W.,Wang, X.,Ying, B.,Zhao, J.,Qi, H.,Zhang, L.
RBD trimer mRNA vaccine elicits broad and protective immune responses against SARS-CoV-2 variants.
Iscience, 25:104043-104043, 2022

PubMed Abstract: With the rapid emergence and spread of SARS-CoV-2 variants, development of vaccines with broad and potent protectivity has become a global priority. Here, we designed a lipid nanoparticle-encapsulated, nucleoside-unmodified mRNA (mRNA-LNP) vaccine encoding the trimerized receptor-binding domain (RBD trimer) and showed its robust capability in inducing broad and protective immune responses against wild-type and major variants of concern (VOCs) in the mouse model of SARS-CoV-2 infection. The protectivity was correlated with RBD-specific B cell responses especially the long-lived plasma B cells in bone marrow, strong ability in triggering BCR clustering, and downstream signaling. Monoclonal antibodies isolated from vaccinated animals demonstrated broad and potent neutralizing activity against VOCs tested. Structure analysis of one representative antibody identified a novel epitope with a high degree of conservation among different variants. Collectively, these results demonstrate that the RBD trimer mRNA vaccine serves as a promising vaccine candidate against SARS-CoV-2 variants and beyond.

PubMed: 35291264
DOI: 10.1016/j.isci.2022.104043

実験手法

ELECTRON MICROSCOPY (3.34 Å)