7FIV

Crystal structure of the complex formed by Wolbachia cytoplasmic incompatibility factors CidA and CidBND1-ND2 from wPip(Tunis)

7FIV の概要

• エントリーDOI: 10.2210/pdb7fiv/pdb
• 分子名称: CidA_I gamma/2 protein, CidB_I b/2 protein (3 entities in total)
• 機能のキーワード: wolbachia, cytoplasmic incompatibility, antitoxin, protein binding
• 由来する生物種: Wolbachia endosymbiont of Culex pipiens; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 146393.93

構造登録者

Xiao, Y.J.,Wang, W.,Chen, X.,Ji, X.Y.,Yang, H.T. (登録日: 2021-08-01, 公開日: 2022-04-06, 最終更新日: 2023-11-29)

主引用文献

Wang, H.,Xiao, Y.,Chen, X.,Zhang, M.,Sun, G.,Wang, F.,Wang, L.,Zhang, H.,Zhang, X.,Yang, X.,Li, W.,Wei, Y.,Yao, D.,Zhang, B.,Li, J.,Cui, W.,Wang, F.,Chen, C.,Shen, W.,Su, D.,Bai, F.,Huang, J.,Ye, S.,Zhang, L.,Ji, X.,Wang, W.,Wang, Z.,Hochstrasser, M.,Yang, H.
Crystal Structures of Wolbachia CidA and CidB Reveal Determinants of Bacteria-induced Cytoplasmic Incompatibility and Rescue.
Nat Commun, 13:1608-1608, 2022

PubMed Abstract: Cytoplasmic incompatibility (CI) results when Wolbachia bacteria-infected male insects mate with uninfected females, leading to embryonic lethality. "Rescue" of viability occurs if the female harbors the same Wolbachia strain. CI is caused by linked pairs of Wolbachia genes called CI factors (CifA and CifB). The co-evolution of CifA-CifB pairs may account in part for the incompatibility patterns documented in insects infected with different Wolbachia strains, but the molecular mechanisms remain elusive. Here, we use X-ray crystallography and AlphaFold to analyze the CI factors from Wolbachia strain wMel called CidA and CidB. Substituting CidA interface residues with those from CidA (from strain wPip) enables the mutant protein to bind CidB and rescue CidB-induced yeast growth defects, supporting the importance of CifA-CifB interaction in CI rescue. Sequence divergence in CidA and CidB proteins affects their pairwise interactions, which may help explain the complex incompatibility patterns of mosquitoes infected with different wPip strains.

PubMed: 35338130
DOI: 10.1038/s41467-022-29273-w

実験手法

X-RAY DIFFRACTION (2.59 Å)