7FFW

The crystal structure of a domain-swapped dimeric maltodextrin-binding protein MalE from Salmonella enterica

7FFW の概要

• エントリーDOI: 10.2210/pdb7ffw/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900030
• 分子名称: Maltodextrin-binding protein, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (6 entities in total)
• 機能のキーワード: domain-swapped, maltodextrin-binding protein, male, hydrolase, sugar binding protein
• 由来する生物種: Salmonella enterica
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 41832.36

構造登録者

Wang, L.,Bu, T.,Bai, X. (登録日: 2021-07-23, 公開日: 2021-09-15, 最終更新日: 2024-05-29)

主引用文献

Wang, L.,Bu, T.,Bai, X.,He, S.,Zhang, J.,Jin, L.,Liu, B.,Dong, Y.,Ha, N.C.,Quan, C.,Nam, K.H.,Xu, Y.
Crystal structure of the domain-swapped dimeric maltodextrin-binding protein MalE from Salmonella enterica.
Acta Crystallogr D Struct Biol, 78:613-622, 2022

PubMed Abstract: MalE is a maltose/maltodextrin-binding protein (MBP) that plays a critical role in most bacterial maltose/maltodextrin-transport systems. Previously reported wild-type MBPs are monomers comprising an N-terminal domain (NTD) and a C-terminal domain (CTD), and maltose-like molecules are recognized between the NTD and CTD and transported to the cell system. Because MBP does not undergo artificial dimerization, it is widely used as a tag for protein expression and purification. Here, the crystal structure of a domain-swapped dimeric MalE from Salmonella enterica (named SeMalE) in complex with maltopentaose is reported for the first time, and its structure is distinct from typical monomeric MalE family members. In the domain-swapped dimer, SeMalE comprises two subdomains: the NTD and CTD. The NTD and CTD of one molecule of SeMalE interact with the CTD and NTD of the partner molecule, respectively. The domain-swapped dimeric conformation was stabilized by interactions between the NTDs, CTDs and linkers from two SeMalE molecules. Additionally, a maltopentaose molecule was found to be located at the interface between the NTD and CTD of different SeMalE molecules. These results provide new insights that will improve the understanding of maltodextrin-binding MalE proteins.

PubMed: 35503209
DOI: 10.1107/S2059798322003114

実験手法

X-RAY DIFFRACTION (1.6 Å)