7FFH

Diarylpentanoid-producing polyketide synthase (N199L mutant)

7FFH の概要

• エントリーDOI: 10.2210/pdb7ffh/pdb
• 分子名称: Type III polyketide synthase (2 entities in total)
• 機能のキーワード: diarylpentanoid, type-iii polyketide synthase, transferase
• 由来する生物種: Aquilaria sinensis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 93689.31

構造登録者

Morita, H.,Wong, C.P.,Liu, Q.,Takeshi, K.,Lee, Y.,Nakashima, Y. (登録日: 2021-07-23, 公開日: 2022-01-19, 最終更新日: 2023-11-29)

主引用文献

Wang, X.H.,Gao, B.W.,Nakashima, Y.,Mori, T.,Zhang, Z.X.,Kodama, T.,Lee, Y.E.,Zhang, Z.K.,Wong, C.P.,Liu, Q.Q.,Qi, B.W.,Wang, J.,Li, J.,Liu, X.,Abe, I.,Morita, H.,Tu, P.F.,Shi, S.P.
Identification of a diarylpentanoid-producing polyketide synthase revealing an unusual biosynthetic pathway of 2-(2-phenylethyl)chromones in agarwood.
Nat Commun, 13:348-348, 2022

PubMed Abstract: 2-(2-Phenylethyl)chromones (PECs) are the principal constituents contributing to the distinctive fragrance of agarwood. How PECs are biosynthesized is currently unknown. In this work, we describe a diarylpentanoid-producing polyketide synthase (PECPS) identified from Aquilaria sinensis. Through biotransformation experiments using fluorine-labeled substrate, transient expression of PECPS in Nicotiana benthamiana, and knockdown of PECPS expression in A. sinensis calli, we demonstrate that the C-C-C scaffold of diarylpentanoid is the common precursor of PECs, and PECPS plays a crucial role in PECs biosynthesis. Crystal structure (1.98 Å) analyses and site-directed mutagenesis reveal that, due to its small active site cavity (247 Å), PECPS employs a one-pot formation mechanism including a "diketide-CoA intermediate-released" step for the formation of the C-C-C scaffold. The identification of PECPS, the pivotal enzyme of PECs biosynthesis, provides insight into not only the feasibility of overproduction of pharmaceutically important PECs using metabolic engineering approaches, but also further exploration of how agarwood is formed.

PubMed: 35039506
DOI: 10.1038/s41467-022-27971-z

実験手法

X-RAY DIFFRACTION (2.2 Å)