7FAU

Structure Determination of the NB1B11-RBD Complex

7FAU の概要

• エントリーDOI: 10.2210/pdb7fau/pdb
• 分子名称: Spike protein S1, NB_1B11, ZINC ION, ... (4 entities in total)
• 機能のキーワード: sars-cov-2, rbd, nanobody, structural protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 76059.82

構造登録者

Shi, Z.Z.,Li, X.X.,Wang, L.,Sun, Z.C.,Zhang, H.W.,Chen, X.C.,Cui, Q.Q.,Qiao, H.R.,Lan, Z.Y.,Zhang, X. (登録日: 2021-07-07, 公開日: 2022-06-01, 最終更新日: 2024-11-20)

主引用文献

Shi, Z.,Li, X.,Wang, L.,Sun, Z.,Zhang, H.,Chen, X.,Cui, Q.,Qiao, H.,Lan, Z.,Zhang, X.,Li, X.,Li, L.,Xu, J.,Gong, R.,Fan, C.,Geng, Y.
Structural basis of nanobodies neutralizing SARS-CoV-2 variants.
Structure, 30:707-720.e5, 2022

PubMed Abstract: Because of the evolutionary variants of SARS-CoV-2, development of broad-spectrum neutralizing antibodies resilient to virus escape is urgently needed. We identified a group of high-affinity nanobodies from camels immunized with receptor-binding domain (RBD) of SARS-CoV-2 spike protein and resolved the structures of two non-competing nanobodies (NB1A7 and NB1B11) in complex with RBD using X-ray crystallography. The structures show that NB1A7 targets the highly conserved cryptic epitope shared by SARS-CoV-2 variants and some other coronaviruses and blocks ACE2 receptor attachment of the spike protein, and NB1B11 epitope overlaps with the contacting surface of ACE2 and is different from the binding site of NB1A7. These two nanobodies were covalently linked into multivalent and bi-paratopic formats, which significantly improved the avidity and neutralization potency and may further inhibit viral escape. The results contribute to the structure-guided design of antibodies against future variants of SARS-CoV-2 virus to combat coronavirus epidemics and pandemics.

PubMed: 35276082
DOI: 10.1016/j.str.2022.02.011

実験手法

X-RAY DIFFRACTION (2.08 Å)