7F5S

human delta-METTL18 60S ribosome

これはPDB形式変換不可エントリーです。

7F5S の概要

• エントリーDOI: 10.2210/pdb7f5s/pdb
• EMDBエントリー: 31465
• 分子名称: 60S ribosomal protein L8, 60S ribosomal protein L7a, 60S ribosomal protein L9, ... (48 entities in total)
• 機能のキーワード: 60s, ribosome
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 45
• 化学式量合計: 2566045.88

構造登録者

Takahashi, M.,Kashiwagi, K.,Ito, T. (登録日: 2021-06-22, 公開日: 2022-06-22)

主引用文献

Matsuura-Suzuki, E.,Shimazu, T.,Takahashi, M.,Kotoshiba, K.,Suzuki, T.,Kashiwagi, K.,Sohtome, Y.,Akakabe, M.,Sodeoka, M.,Dohmae, N.,Ito, T.,Shinkai, Y.,Iwasaki, S.
METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance.
Elife, 11:-, 2022

PubMed Abstract: Protein methylation occurs predominantly on lysine and arginine residues, but histidine also serves as a methylation substrate. However, a limited number of enzymes responsible for this modification have been reported. Moreover, the biological role of histidine methylation has remained poorly understood to date. Here, we report that human METTL18 is a histidine methyltransferase for the ribosomal protein RPL3 and that the modification specifically slows ribosome traversal on Tyr codons, allowing the proper folding of synthesized proteins. By performing an in vitro methylation assay with a methyl donor analog and quantitative mass spectrometry, we found that His245 of RPL3 is methylated at the τ- position by METTL18. Structural comparison of the modified and unmodified ribosomes showed stoichiometric modification and suggested a role in translation reactions. Indeed, genome-wide ribosome profiling and an in vitro translation assay revealed that translation elongation at Tyr codons was suppressed by RPL3 methylation. Because the slower elongation provides enough time for nascent protein folding, RPL3 methylation protects cells from the cellular aggregation of Tyr-rich proteins. Our results reveal histidine methylation as an example of a ribosome modification that ensures proteome integrity in cells.

PubMed: 35674491
DOI: 10.7554/eLife.72780

実験手法

ELECTRON MICROSCOPY (2.72 Å)