7F2R

Crystal structure of VinK-VinL covalent complex formed with a pantetheineamide cross-linking probe

7F2R の概要

• エントリーDOI: 10.2210/pdb7f2r/pdb
• 分子名称: Malonyl-CoA-[acyl-carrier-protein] transacylase, Acyl-carrier-protein, N-[2-(acetylamino)ethyl]-N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alaninamide, ... (4 entities in total)
• 機能のキーワード: acyl carrier protein, polyketide biosynthesis, transferase
• 由来する生物種: Streptomyces halstedii; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 93110.82

構造登録者

Miyanaga, A.,Ouchi, R.,Kudo, F.,Eguchi, T. (登録日: 2021-06-14, 公開日: 2021-09-01, 最終更新日: 2024-11-06)

主引用文献

Miyanaga, A.,Ouchi, R.,Kudo, F.,Eguchi, T.
Complex structure of the acyltransferase VinK and the carrier protein VinL with a pantetheine cross-linking probe.
Acta Crystallogr.,Sect.F, 77:294-302, 2021

PubMed Abstract: Acyltransferases are responsible for the selection and loading of acyl units onto carrier proteins in polyketide and fatty-acid biosynthesis. Despite the importance of protein-protein interactions between the acyltransferase and the carrier protein, structural information on acyltransferase-carrier protein interactions is limited because of the transient interactions between them. In the biosynthesis of the polyketide vicenistatin, the acyltransferase VinK recognizes the carrier protein VinL for the transfer of a dipeptidyl unit. The crystal structure of a VinK-VinL covalent complex formed with a 1,2-bismaleimidoethane cross-linking reagent has been determined previously. Here, the crystal structure of a VinK-VinL covalent complex formed with a pantetheine cross-linking probe is reported at 1.95 Å resolution. In the structure of the VinK-VinL-probe complex, the pantetheine probe that is attached to VinL is covalently connected to the side chain of the mutated Cys106 of VinK. The interaction interface between VinK and VinL is essentially the same in the two VinK-VinL complex structures, although the position of the pantetheine linker slightly differs. This structural observation suggests that interface interactions are not affected by the cross-linking strategy used.

PubMed: 34473106
DOI: 10.1107/S2053230X21008761

実験手法

X-RAY DIFFRACTION (1.95 Å)