7F1L

Designed enzyme RA61 M48K/I72D mutant: form V

7F1L の概要

• エントリーDOI: 10.2210/pdb7f1l/pdb
• 分子名称: Engineered Retroaldolase, IMIDAZOLE, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: aldol reaction, retro-aldol reaction, mutation, reaction direction, de novo protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22291.62

構造登録者

Fujioka, T.,Oka, M.,Numoto, N.,Ito, N.,Oda, M.,Tanaka, F. (登録日: 2021-06-09, 公開日: 2021-11-24, 最終更新日: 2023-11-29)

主引用文献

Fujioka, T.,Numoto, N.,Akama, H.,Shilpa, K.,Oka, M.,Roy, P.K.,Krishna, Y.,Ito, N.,Baker, D.,Oda, M.,Tanaka, F.
Varying the Directionality of Protein Catalysts for Aldol and Retro-Aldol Reactions.
Chembiochem, 23:e202100435-e202100435, 2022

PubMed Abstract: Natural aldolase enzymes and created retro-aldolase protein catalysts often catalyze both aldol and retro-aldol reactions depending on the concentrations of the reactants and the products. Here, we report that the directionality of protein catalysts can be altered by replacing one amino acid. The protein catalyst derived from a scaffold of a previously reported retro-aldolase catalyst, catalyzed aldol reactions more efficiently than the previously reported retro-aldolase catalyst. The retro-aldolase catalyst efficiently catalyzed the retro-aldol reaction but was less efficient in catalyzing the aldol reaction. The results indicate that protein catalysts with varying levels of directionality in usually reversibly catalyzed aldol and retro-aldol reactions can be generated from the same protein scaffold.

PubMed: 34698422
DOI: 10.1002/cbic.202100435

実験手法

X-RAY DIFFRACTION (1.7 Å)