7EXY

T-hairpin structure found in the RNA element involved in the piRNA biogenesis

Summary for 7EXY

• Entry DOI: 10.2210/pdb7exy/pdb
• NMR Information: BMRB: 36422
• Descriptor: RNA (5'-R(*UP*UP*GP*GP*UP*UP*UP*GP*AP*UP*UP*CP*CP*AP*AP*G)-3') (1 entity in total)
• Functional Keywords: pirna, cis element, rna
• Biological source: Drosophila melanogaster
• Total number of polymer chains: 1
• Total formula weight: 5077.01

Authors

Takase, N.,Kawai, G. (deposition date: 2021-05-29, release date: 2022-03-02, Last modification date: 2024-05-15)

Primary citation

Takase, N.,Otsu, M.,Hirakata, S.,Ishizu, H.,Siomi, M.C.,Kawai, G.
T-hairpin structure found in the RNA element involved in piRNA biogenesis.
Rna, 28:541-550, 2022

PubMed Abstract: PIWI-interacting RNAs (piRNAs) repress transposons to protect the germline genome from DNA damage caused by transposon transposition. In , the () mRNA is consumed to produce piRNA in its 3'-UTR. A element located within the 3'-UTR, , is necessary for piRNA biogenesis. In this study, we analyzed the structure of the RNA, a 100-nt RNA corresponding to the element, by the SHAPE and NMR analyses and found that a stable hairpin structure formed in the 5' half of the RNA. The tertiary structure of the 16-nt stable hairpin was analyzed by NMR, and a novel stem-loop structure, the T-hairpin, was found. In the T-hairpin, four uridine residues are exposed to the solvent, suggesting that this stem-loop is the target of Yb protein, a Tudor domain-containing piRNA biogenesis factor. The piRNA biogenesis assay showed that both the T-hairpin and the 3' half are required for the function of the element, suggesting that both the T-hairpin and the 3' half are recognized by Yb protein.

PubMed: 34987083
DOI: 10.1261/rna.078967.121

Experimental method

SOLUTION NMR