7EW6

Barley photosystem I-LHCI-Lhca5 supercomplex

Summary for 7EW6

• Entry DOI: 10.2210/pdb7ew6/pdb
• EMDB information: 31348
• Descriptor: Photosystem I P700 chlorophyll a apoprotein A1, Photosystem I reaction center subunit psaK, chloroplastic, Photosystem I reaction center subunit XI, chloroplastic, ... (26 entities in total)
• Functional Keywords: photosystem i, light-harvesting complex, lhca5, photosynthesis
• Biological source: Hordeum vulgare subsp. spontaneum; More
• Total number of polymer chains: 15
• Total formula weight: 570497.79

Authors

Wang, W.D.,Shen, L.,Tang, K.,Han, G.Y.,Zhang, X.,Shen, J.R. (deposition date: 2021-05-24, release date: 2021-12-22, Last modification date: 2024-11-13)

Primary citation

Shen, L.,Tang, K.,Wang, W.,Wang, C.,Wu, H.,Mao, Z.,An, S.,Chang, S.,Kuang, T.,Shen, J.R.,Han, G.,Zhang, X.
Architecture of the chloroplast PSI-NDH supercomplex in Hordeum vulgare.
Nature, 601:649-654, 2022

PubMed Abstract: The chloroplast NADH dehydrogenase-like (NDH) complex is composed of at least 29 subunits and has an important role in mediating photosystem I (PSI) cyclic electron transport (CET). The NDH complex associates with PSI to form the PSI-NDH supercomplex and fulfil its function. Here, we report cryo-electron microscopy structures of a PSI-NDH supercomplex from barley (Hordeum vulgare). The structures reveal that PSI-NDH is composed of two copies of the PSI-light-harvesting complex I (LHCI) subcomplex and one NDH complex. Two monomeric LHCI proteins, Lhca5 and Lhca6, mediate the binding of two PSI complexes to NDH. Ten plant chloroplast-specific NDH subunits are presented and their exact positions as well as their interactions with other subunits in NDH are elucidated. In all, this study provides a structural basis for further investigations on the functions and regulation of PSI-NDH-dependent CET.

PubMed: 34879391
DOI: 10.1038/s41586-021-04277-6

Experimental method

ELECTRON MICROSCOPY (3.4 Å)