7ETF

Crystal structure of AbHpaI-Mn-pyruvate-succinic semialdehyde complex, Class II aldolase, HpaI from Acinetobacter baumannii

7ETF の概要

• エントリーDOI: 10.2210/pdb7etf/pdb
• 分子名称: 4-hydroxy-2-oxoheptanedioate aldolase, PYRUVIC ACID, MANGANESE (II) ION, ... (6 entities in total)
• 機能のキーワード: tim barrel, aldehyde lyase, lyase
• 由来する生物種: Acinetobacter baumannii
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 87043.07

構造登録者

Watthaisong, P.,Binlaeh, A.,Jaruwat, A.,Chaiyen, P.,Chitnumsub, P.,Maenpuen, S. (登録日: 2021-05-12, 公開日: 2021-11-03, 最終更新日: 2023-11-29)

主引用文献

Watthaisong, P.,Binlaeh, A.,Jaruwat, A.,Lawan, N.,Tantipisit, J.,Jaroensuk, J.,Chuaboon, L.,Phonbuppha, J.,Tinikul, R.,Chaiyen, P.,Chitnumsub, P.,Maenpuen, S.
Catalytic and structural insights into a stereospecific and thermostable Class II aldolase HpaI from Acinetobacter baumannii.
J.Biol.Chem., 297:101280-101280, 2021

PubMed Abstract: Aldolases catalyze the reversible reactions of aldol condensation and cleavage and have strong potential for the synthesis of chiral compounds, widely used in pharmaceuticals. Here, we investigated a new Class II metal aldolase from the p-hydroxyphenylacetate degradation pathway in Acinetobacter baumannii, 4-hydroxy-2-keto-heptane-1,7-dioate aldolase (AbHpaI), which has various properties suitable for biocatalysis, including stereoselectivity/stereospecificity, broad aldehyde utilization, thermostability, and solvent tolerance. Notably, the use of Zn by AbHpaI as a native cofactor is distinct from other enzymes in this class. AbHpaI can also use other metal ion (M) cofactors, except Ca, for catalysis. We found that Zn yielded the highest enzyme complex thermostability (T of 87 °C) and solvent tolerance. All AbHpaI•M complexes demonstrated preferential cleavage of (4R)-2-keto-3-deoxy-D-galactonate ((4R)-KDGal) over (4S)-2-keto-3-deoxy-D-gluconate ((4S)-KDGlu), with AbHpaI•Zn displaying the highest R/S stereoselectivity ratio (sixfold higher than other M cofactors). For the aldol condensation reaction, AbHpaI•M only specifically forms (4R)-KDGal and not (4S)-KDGlu and preferentially catalyzes condensation rather than cleavage by ∼40-fold. Based on 11 X-ray structures of AbHpaI complexed with M and ligands at 1.85 to 2.0 Å resolution, the data clearly indicate that the M cofactors form an octahedral geometry with Glu151 and Asp177, pyruvate, and water molecules. Moreover, Arg72 in the Zn-bound form governs the stereoselectivity/stereospecificity of AbHpaI. X-ray structures also show that Ca binds at the trimer interface via interaction with Asp51. Hence, we conclude that AbHpaI•Zn is distinctive from its homologues in substrate stereospecificity, preference for aldol formation over cleavage, and protein robustness, and is attractive for biocatalytic applications.

PubMed: 34624314
DOI: 10.1016/j.jbc.2021.101280

実験手法

X-RAY DIFFRACTION (2 Å)