7ET3

C5 portal vertex in the enveloped virion capsid

7ET3 の概要

• エントリーDOI: 10.2210/pdb7et3/pdb
• EMDBエントリー: 31297
• 分子名称: Triplex capsid protein 2, Large tegument protein deneddylase, Triplex capsid protein 1, ... (8 entities in total)
• 機能のキーワード: c5 portal vertex, enveloped capsid, viral protein
• 由来する生物種: Human cytomegalovirus (HHV-5, Human herpesvirus 5); 詳細
• タンパク質・核酸の鎖数: 23
• 化学式量合計: 2002476.33

構造登録者

Li, Z.,Yu, X. (登録日: 2021-05-12, 公開日: 2021-07-14, 最終更新日: 2025-07-02)

主引用文献

Li, Z.,Pang, J.,Dong, L.,Yu, X.
Structural basis for genome packaging, retention, and ejection in human cytomegalovirus.
Nat Commun, 12:4538-4538, 2021

PubMed Abstract: How the human cytomegalovirus (HCMV) genome-the largest among human herpesviruses-is packaged, retained, and ejected remains unclear. We present the in situ structures of the symmetry-mismatched portal and the capsid vertex-specific components (CVSCs) of HCMV. The 5-fold symmetric 10-helix anchor-uncommon among known portals-contacts the portal-encircling DNA, which is presumed to squeeze the portal as the genome packaging proceeds. We surmise that the 10-helix anchor dampens this action to delay the portal reaching a "head-full" packaging state, thus facilitating the large genome to be packaged. The 6-fold symmetric turret, latched via a coiled coil to a helix from a major capsid protein, supports the portal to retain the packaged genome. CVSCs at the penton vertices-presumed to increase inner capsid pressure-display a low stoichiometry, which would aid genome retention. We also demonstrate that the portal and capsid undergo conformational changes to facilitate genome ejection after viral cell entry.

PubMed: 34315863
DOI: 10.1038/s41467-021-24820-3

実験手法

ELECTRON MICROSCOPY (4.2 Å)