7ET0

Crystal structure of the complex formed by Wolbachia cytoplasmic incompatibility factors CinA and CinB from wPip

7ET0 の概要

• エントリーDOI: 10.2210/pdb7et0/pdb
• 分子名称: Bacteria factor B, Bacteria factor A (3 entities in total)
• 機能のキーワード: wolbachia, nuclease, antitoxin, toxin, protein binding
• 由来する生物種: Wolbachia pipientis subsp. Culex pipiens (strain wPip); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 273855.94

構造登録者

Xiao, Y.J.,Wang, W.,Chen, X.,Ji, X.Y.,Yang, H.T. (登録日: 2021-05-12, 公開日: 2021-11-10, 最終更新日: 2024-05-29)

主引用文献

Xiao, Y.,Chen, H.,Wang, H.,Zhang, M.,Chen, X.,Berk, J.M.,Zhang, L.,Wei, Y.,Li, W.,Cui, W.,Wang, F.,Wang, Q.,Cui, C.,Li, T.,Chen, C.,Ye, S.,Zhang, L.,Ji, X.,Huang, J.,Wang, W.,Wang, Z.,Hochstrasser, M.,Yang, H.
Structural and mechanistic insights into the complexes formed by Wolbachia cytoplasmic incompatibility factors.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: bacteria, inherited through the female germ line, infect a large fraction of arthropod species. Many strains manipulate host reproduction, most commonly through cytoplasmic incompatibility (CI). CI, a conditional male sterility, results when -infected male insects mate with uninfected females; viability is restored if the female is similarly infected (called "rescue"). CI is used to help control mosquito-borne viruses such as dengue and Zika, but its mechanisms remain unknown. The coexpressed CI factors CifA and CifB form stable complexes in vitro, but the timing and function of this interaction in the insect are unresolved. CifA expression in the female germ line is sufficient for rescue. We report high-resolution structures of a CI-factor complex, CinA-CinB, which utilizes a unique binding mode between the CinA rescue factor and the CinB nuclease; the structures were validated by biochemical and yeast growth analyses. Importantly, transgenic expression in of a nonbinding CinA mutant, designed based on the CinA-CinB structure, suggests CinA expressed in females must bind CinB imported by sperm in order to rescue embryonic viability. Binding between cognate factors is conserved in an enzymatically distinct CI system, CidA-CidB, suggesting universal features in CI induction and rescue.

PubMed: 34620712
DOI: 10.1073/pnas.2107699118

実験手法

X-RAY DIFFRACTION (2.2 Å)