7ESD

Mature Donggang virus

7ESD の概要

• エントリーDOI: 10.2210/pdb7esd/pdb
• EMDBエントリー: 31288
• 分子名称: Genome polyprotein (2 entities in total)
• 機能のキーワード: flavivirus mature, virus
• 由来する生物種: Donggang virus; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 189105.43

構造登録者

Zhang, Y.,Liang, D. (登録日: 2021-05-10, 公開日: 2022-05-18, 最終更新日: 2024-10-16)

主引用文献

Zhang, Y.,Liang, D.,Yuan, F.,Yan, Y.,Wang, Z.,Liu, P.,Yu, Q.,Zhang, X.,Wang, X.,Zheng, A.
Replication is the key barrier during the dual-host adaptation of mosquito-borne flaviviruses.
Proc.Natl.Acad.Sci.USA, 119:e2110491119-e2110491119, 2022

PubMed Abstract: Mosquito-borne flaviviruses (MBFs) adapt to a dual-host transmission circle between mosquitoes and vertebrates. Dual-host affiliated insect-specific flaviviruses (dISFs), discovered from mosquitoes, are phylogenetically similar to MBFs but do not infect vertebrates. Thus, dISF–MBF chimeras could be an ideal model to study the dual-host adaptation of MBFs. Using the pseudoinfectious reporter virus particle and reverse genetics systems, we found dISFs entered vertebrate cells as efficiently as the MBFs but failed to initiate replication. Exchange of the untranslational regions (UTRs) of Donggang virus (DONV), a dISF, with those from Zika virus (ZIKV) rescued DONV replication in vertebrate cells, and critical secondary RNA structures were further mapped. Essential UTR-binding host factors were screened for ZIKV replication in vertebrate cells, displaying different binding patterns. Therefore, our data demonstrate a post-entry cross-species transmission mechanism of MBFs, while UTR-host interaction is critical for dual-host adaptation.

PubMed: 35294288
DOI: 10.1073/pnas.2110491119

実験手法

ELECTRON MICROSCOPY (3.9 Å)