7EQI

ChlB3 [Aceyltransferase]

7EQI の概要

• エントリーDOI: 10.2210/pdb7eqi/pdb
• 分子名称: ChlB3 (2 entities in total)
• 機能のキーワード: acyltransferase, chlb3, transferase
• 由来する生物種: Streptomyces antibioticus
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 296143.94

構造登録者

Saeed, A.U.,Zheng, J. (登録日: 2021-05-02, 公開日: 2021-06-09, 最終更新日: 2023-11-29)

主引用文献

Saeed, A.U.,Rahman, M.U.,Chen, H.F.,Zheng, J.
Structural Insight of KSIII ( beta-Ketoacyl-ACP Synthase)-like Acyltransferase ChlB3 in the Biosynthesis of Chlorothricin.
Molecules, 27:-, 2022

PubMed Abstract: Chlorothricin (CHL) belongs to a spirotetronate antibiotic family produced by that inhibits pyruvate carboxylase and malate dehydrogenase. For the biosynthesis of CHL, ChlB3 plays a crucial role by introducing the 6-methylsalicylic acid (6MSA) moiety to ChlB2, an acyl carrier protein (ACP). However, the structural insight and catalytic mechanism of ChlB3 was unclear. In the current study, the crystal structure of ChlB3 was solved at 3.1 Å-resolution and a catalytic mechanism was proposed on the basis of conserved residues of structurally related enzymes. ChlB3 is a dimer having the same active sites as CerJ (a structural homologous enzyme) and uses a KSIII-like fold to work as an acyltransferase. The relaxed substrate specificity of ChlB3 was defined by its catalytic efficiencies (/) for non-ACP tethered synthetic substrates such as 6MSA-SNAC, acetyl-SNAC, and cyclohexonyl-SNAC. ChlB3 successfully detached the 6MSA moiety from 6MSA-SNAC substrate and this hydrolytic activity demonstrated that ChlB3 has the potential to catalyze non-ACP tethered substrates. Structural comparison indicated that ChlB3 belongs to FabH family and showed 0.6-2.5 Å root mean square deviation (RMSD) with structural homologous enzymes. Molecular docking and dynamics simulations were implemented to understand substrate active site and structural behavior such as the open and closed conformation of the ChlB3 protein. The resultant catalytic and substrate recognition mechanism suggested that ChlB3 has the potential to use non-native substrates and minimize the labor of expressing ACP protein. This versatile acyltransferase activity may pave the way for manufacturing CHL variants and may help to hydrolyze several thioester-based compounds.

PubMed: 36234941
DOI: 10.3390/molecules27196405

実験手法

X-RAY DIFFRACTION (3.1 Å)