7EQG

Structure of Csy-AcrIF5

7EQG の概要

• エントリーDOI: 10.2210/pdb7eqg/pdb
• EMDBエントリー: 31265
• 分子名称: CRISPR type I-F/YPEST-associated protein Csy2, CRISPR-associated protein Csy3, AcrIF5, ... (8 entities in total)
• 機能のキーワード: complex, inhibitor, immune system, immune system-rna-dna complex, immune system/rna/dna
• 由来する生物種: Pseudomonas aeruginosa; 詳細
• タンパク質・核酸の鎖数: 17
• 化学式量合計: 427934.73

構造登録者

Zhang, L.,Feng, Y. (登録日: 2021-05-01, 公開日: 2022-03-09, 最終更新日: 2024-06-05)

主引用文献

Xie, Y.,Zhang, L.,Gao, Z.,Yin, P.,Wang, H.,Li, H.,Chen, Z.,Zhang, Y.,Yang, M.,Feng, Y.
AcrIF5 specifically targets DNA-bound CRISPR-Cas surveillance complex for inhibition.
Nat.Chem.Biol., 18:670-677, 2022

PubMed Abstract: CRISPR-Cas systems are prokaryotic antiviral systems, and phages use anti-CRISPR proteins (Acrs) to inactivate these systems. Here we present structural and functional analyses of AcrIF5, exploring its unique anti-CRISPR mechanism. AcrIF5 shows binding specificity only for the target DNA-bound form of the crRNA-guided surveillance (Csy) complex, but not the apo Csy complex from the type I-F CRISPR-Cas system. We solved the structure of the Csy-dsDNA-AcrIF5 complex, revealing that the conformational changes of the Csy complex caused by dsDNA binding dictate the binding specificity for the Csy-dsDNA complex by AcrIF5. Mechanistically, five AcrIF5 molecules bind one Csy-dsDNA complex, which destabilizes the helical bundle domain of Cas8f, thus preventing subsequent Cas2/3 recruitment. AcrIF5 exists in symbiosis with AcrIF3, which blocks Cas2/3 recruitment. This attack on the recruitment event stands in contrast to the conventional mechanisms of blocking binding of target DNA. Overall, our study reveals an unprecedented mechanism of CRISPR-Cas inhibition by AcrIF5.

PubMed: 35301482
DOI: 10.1038/s41589-022-00995-8

実験手法

ELECTRON MICROSCOPY (3.2 Å)