7EPP

Recombinant Alfalfa Mosaic virus coat protein virus-like particle (rAMV-CP VLP)

7EPP の概要

• エントリーDOI: 10.2210/pdb7epp/pdb
• EMDBエントリー: 31248
• 分子名称: Capsid protein (2 entities in total)
• 機能のキーワード: alfalfa mosaic virus, virus-like particle, virus like particle
• 由来する生物種: Alfalfa mosaic virus (strain 425 / isolate Madison)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28373.33

構造登録者

Jeong, H.,Lee, S. (登録日: 2021-04-27, 公開日: 2021-11-17, 最終更新日: 2024-06-05)

主引用文献

Jeong, H.,Park, Y.,Song, S.,Min, K.,Woo, J.S.,Lee, Y.H.,Sohn, E.J.,Lee, S.
Characterization of alfalfa mosaic virus capsid protein using Cryo-EM.
Biochem.Biophys.Res.Commun., 559:161-167, 2021

PubMed Abstract: VLPs are virus-like particles that comprise viral capsid proteins that can self-assemble and mimic the shape and size of real viral particles; however, because they do not contain genetic material they cannot infect host cells. VLPs have great potential as safe drug/vehicle candidates; therefore, they are gaining popularity in the field of preventive medicine and therapeutics. Indeed, extensive studies are underway to examine their role as carriers for immunization and as vehicles for delivery of therapeutic agents. Here, we examined the possibility of developing VLP-utilizing technology based on an efficient VLP production process and high-resolution structural analysis. Nicotiana benthamiana was used as an expression platform to produce the coat protein of the alfalfa mosaic virus (AMV-CP). About 250 mg/kg of rAMV-CP was produced from Nicotiana benthamiana leaves. Structural analysis revealed that the oligomeric status of rAMV-CP changed according to the composition and pH of the buffer. Size exclusion chromatography and electron microscopy analysis confirmed the optimal conditions for rAMV-CP VLP formation, and a 2.4 Å resolution structure was confirmed by cryo-EM analysis. Based on the efficient protein production, VLP manufacturing technology, and high-resolution structure presented herein, we suggest that rAMV-CP VLP is a useful platform for development of various new drugs.

PubMed: 33940388
DOI: 10.1016/j.bbrc.2021.04.060

実験手法

ELECTRON MICROSCOPY (2.4 Å)