7EN0

Structure and Activity of SLAC1 Channels for Stomatal Signaling in Leaves

7EN0 の概要

• エントリーDOI: 10.2210/pdb7en0/pdb
• EMDBエントリー: 31197
• 分子名称: SLow Anion Channel 1, DIUNDECYL PHOSPHATIDYL CHOLINE, SPHINGOSINE (3 entities in total)
• 機能のキーワード: anion channel, membrane protein
• 由来する生物種: Brachypodium distachyon (Purple false brome, Trachynia distachya)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 190802.02

構造登録者

Deng, Y.,Kashtoh, H.,Wang, Q.,Zhen, G.,Li, Q.,Tang, L.,Gao, H.,Zhang, C.,Qin, L.,Su, M.,Li, F.,Huang, X.,Wang, Y.,Xie, Q.,Clarke, O.B.,Hendrickson, W.A.,Chen, Y. (登録日: 2021-04-15, 公開日: 2021-05-19, 最終更新日: 2024-06-05)

主引用文献

Deng, Y.N.,Kashtoh, H.,Wang, Q.,Zhen, G.X.,Li, Q.Y.,Tang, L.H.,Gao, H.L.,Zhang, C.R.,Qin, L.,Su, M.,Li, F.,Huang, X.H.,Wang, Y.C.,Xie, Q.,Clarke, O.B.,Hendrickson, W.A.,Chen, Y.H.
Structure and activity of SLAC1 channels for stomatal signaling in leaves.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: Stomata in leaves regulate gas exchange between the plant and its atmosphere. Various environmental stimuli elicit abscisic acid (ABA); ABA leads to phosphoactivation of slow anion channel 1 (SLAC1); SLAC1 activity reduces turgor pressure in aperture-defining guard cells; and stomatal closure ensues. We used electrophysiology for functional characterizations of SLAC1 (SLAC1) and cryoelectron microscopy (cryo-EM) for structural analysis of SLAC1 (SLAC1), at 2.97-Å resolution. We identified 14 phosphorylation sites in SLAC1 and showed nearly 330-fold channel-activity enhancement with 4 to 6 of these phosphorylated. Seven SLAC1-conserved arginines are poised in SLAC1 for regulatory interaction with the N-terminal extension. This SLAC1 structure has its pores closed, in a basal state, spring loaded by phenylalanyl residues in high-energy conformations. SLAC1 phosphorylation fine-tunes an equilibrium between basal and activated SLAC1 trimers, thereby controlling the degree of stomatal opening.

PubMed: 33926963
DOI: 10.1073/pnas.2015151118

実験手法

ELECTRON MICROSCOPY (2.97 Å)