7EMO

Crystal Structure of HasAp Capturing Iron Tetraphenylporphyrin

7EMO の概要

• エントリーDOI: 10.2210/pdb7emo/pdb
• 分子名称: Heme acquisition protein HasAp, [5,10,15,20-tetraphenylporphyrinato(2-)-kappa~4~N~21~,N~22~,N~23~,N~24~]iron, 3-CYCLOHEXYL-1-PROPYLSULFONIC ACID, ... (7 entities in total)
• 機能のキーワード: heme acquisition protein, transport protein
• 由来する生物種: Pseudomonas aeruginosa str. PAO1
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 60710.54

構造登録者

Shisaka, Y.,Sugimoto, H.,Shoji, O. (登録日: 2021-04-14, 公開日: 2022-04-20, 最終更新日: 2023-11-29)

主引用文献

Shisaka, Y.,Sakakibara, E.,Suzuki, K.,Stanfield, J.K.,Onoda, H.,Ueda, G.,Hatano, M.,Sugimoto, H.,Shoji, O.
Tetraphenylporphyrin Enters the Ring: First Example of a Complex between Highly Bulky Porphyrins and a Protein.
Chembiochem, 23:e202200095-e202200095, 2022

PubMed Abstract: Tetraphenylporphyrin (TPP) is a symmetrically substituted synthetic porphyrin whose properties can be readily modified, providing it with significant advantages over naturally occurring porphyrins. Herein, we report the first example of a stable complex between a native biomolecule, the haemoprotein HasA, and TPP as well as its derivatives. The X-ray crystal structures of nine different HasA-TPP complexes were solved at high resolutions. HasA capturing TPP derivatives was also demonstrated to inhibit growth of the opportunistic pathogen Pseudomonas aeruginosa. Mutant variants of HasA binding FeTPP were shown to possess a different mode of coordination, permitting the cyclopropanation of styrene.

PubMed: 35352458
DOI: 10.1002/cbic.202200095

実験手法

X-RAY DIFFRACTION (1.5 Å)