7ELL

In situ structure of capping enzyme lambda2, penetration protein mu1 of mammalian reovirus capsid asymmetric unit.

7ELL の概要

• エントリーDOI: 10.2210/pdb7ell/pdb
• EMDBエントリー: 31184
• 分子名称: Mu1, mRNA (guanine-N(7)-)-methyltransferase, MYRISTIC ACID, ... (4 entities in total)
• 機能のキーワード: mammalian reovirus 3, capping enzyme lambda2, penetration protein mu1, virus, viral protein-transferase complex, viral protein/transferase
• 由来する生物種: Mammalian orthoreovirus 3; 詳細
• タンパク質・核酸の鎖数: 21
• 化学式量合計: 909073.93

構造登録者

Zhou, Z.H.,Pan, M. (登録日: 2021-04-12, 公開日: 2021-10-20, 最終更新日: 2024-11-06)

主引用文献

Pan, M.,Alvarez-Cabrera, A.L.,Kang, J.S.,Wang, L.,Fan, C.,Zhou, Z.H.
Asymmetric reconstruction of mammalian reovirus reveals interactions among RNA, transcriptional factor mu2 and capsid proteins.
Nat Commun, 12:4176-4176, 2021

PubMed Abstract: Mammalian reovirus (MRV) is the prototypical member of genus Orthoreovirus of family Reoviridae. However, lacking high-resolution structures of its RNA polymerase cofactor μ2 and infectious particle, limits understanding of molecular interactions among proteins and RNA, and their contributions to virion assembly and RNA transcription. Here, we report the 3.3 Å-resolution asymmetric reconstruction of transcribing MRV and in situ atomic models of its capsid proteins, the asymmetrically attached RNA-dependent RNA polymerase (RdRp) λ3, and RdRp-bound nucleoside triphosphatase μ2 with a unique RNA-binding domain. We reveal molecular interactions among virion proteins and genomic and messenger RNA. Polymerase complexes in three Spinoreovirinae subfamily members are organized with different pseudo-D symmetries to engage their highly diversified genomes. The above interactions and those between symmetry-mismatched receptor-binding σ1 trimers and RNA-capping λ2 pentamers balance competing needs of capsid assembly, external protein removal, and allosteric triggering of endogenous RNA transcription, before, during and after infection, respectively.

PubMed: 34234134
DOI: 10.1038/s41467-021-24455-4

実験手法

ELECTRON MICROSCOPY (3.8 Å)