7EKF

Structure of SARS-CoV-2 Alpha variant spike receptor-binding domain complexed with human ACE2

7EKF の概要

• エントリーDOI: 10.2210/pdb7ekf/pdb
• 分子名称: Angiotensin-converting enzyme 2, Spike protein S1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: sars-cov-2, spike, rbd, 501y.v1, ace2, viral protein, hydrolase-viral protein complex, hydrolase/viral protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 97357.49

構造登録者

Han, P.C.,Su, C.,Zhang, Y.F.,Qi, J.X.,Gao, G.F. (登録日: 2021-04-05, 公開日: 2021-11-03, 最終更新日: 2023-11-29)

主引用文献

Han, P.,Su, C.,Zhang, Y.,Bai, C.,Zheng, A.,Qiao, C.,Wang, Q.,Niu, S.,Chen, Q.,Zhang, Y.,Li, W.,Liao, H.,Li, J.,Zhang, Z.,Cho, H.,Yang, M.,Rong, X.,Hu, Y.,Huang, N.,Yan, J.,Wang, Q.,Zhao, X.,Gao, G.F.,Qi, J.
Molecular insights into receptor binding of recent emerging SARS-CoV-2 variants.
Nat Commun, 12:6103-6103, 2021

PubMed Abstract: Multiple SARS-CoV-2 variants of concern (VOCs) have been emerging and some have been linked to an increase in case numbers globally. However, there is yet a lack of understanding of the molecular basis for the interactions between the human ACE2 (hACE2) receptor and these VOCs. Here we examined several VOCs including Alpha, Beta, and Gamma, and demonstrate that five variants receptor-binding domain (RBD) increased binding affinity for hACE2, and four variants pseudoviruses increased entry into susceptible cells. Crystal structures of hACE2-RBD complexes help identify the key residues facilitating changes in hACE2 binding affinity. Additionally, soluble hACE2 protein efficiently prevent most of the variants pseudoviruses. Our findings provide important molecular information and may help the development of novel therapeutic and prophylactic agents targeting these emerging mutants.

PubMed: 34671049
DOI: 10.1038/s41467-021-26401-w

実験手法

X-RAY DIFFRACTION (2.85 Å)