7EJ7

Yeast Dmc1 post-synaptic complex

7EJ7 の概要

• エントリーDOI: 10.2210/pdb7ej7/pdb
• EMDBエントリー: 31154
• 分子名称: HLJ1_G0016300.mRNA.1.CDS.1, DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), DNA (5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*A)-3'), ... (5 entities in total)
• 機能のキーワード: meiotic recombination protein dmc1, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 117033.76

構造登録者

Zhao, L.Y.,Xu, J.F.,Wang, H.W. (登録日: 2021-04-01, 公開日: 2022-04-20, 最終更新日: 2024-06-05)

主引用文献

Xu, J.,Zhao, L.,Peng, S.,Chu, H.,Liang, R.,Tian, M.,Connell, P.P.,Li, G.,Chen, C.,Wang, H.W.
Mechanisms of distinctive mismatch tolerance between Rad51 and Dmc1 in homologous recombination.
Nucleic Acids Res., 49:13135-13149, 2021

PubMed Abstract: Homologous recombination (HR) is a primary DNA double-strand breaks (DSBs) repair mechanism. The recombinases Rad51 and Dmc1 are highly conserved in the RecA family; Rad51 is mainly responsible for DNA repair in somatic cells during mitosis while Dmc1 only works during meiosis in germ cells. This spatiotemporal difference is probably due to their distinctive mismatch tolerance during HR: Rad51 does not permit HR in the presence of mismatches, whereas Dmc1 can tolerate certain mismatches. Here, the cryo-EM structures of Rad51-DNA and Dmc1-DNA complexes revealed that the major conformational differences between these two proteins are located in their Loop2 regions, which contain invading single-stranded DNA (ssDNA) binding residues and double-stranded DNA (dsDNA) complementary strand binding residues, stabilizing ssDNA and dsDNA in presynaptic and postsynaptic complexes, respectively. By combining molecular dynamic simulation and single-molecule FRET assays, we identified that V273 and D274 in the Loop2 region of human RAD51 (hRAD51), corresponding to P274 and G275 of human DMC1 (hDMC1), are the key residues regulating mismatch tolerance during strand exchange in HR. This HR accuracy control mechanism provides mechanistic insights into the specific roles of Rad51 and Dmc1 in DNA double-strand break repair and may shed light on the regulatory mechanism of genetic recombination in mitosis and meiosis.

PubMed: 34871438
DOI: 10.1093/nar/gkab1141

実験手法

ELECTRON MICROSCOPY (3.41 Å)