7EGA

TFIID-based intermediate PIC on PUMA promoter

Summary for 7EGA

• Entry DOI: 10.2210/pdb7ega/pdb
• EMDB information: 31110
• Descriptor: Transcription initiation factor TFIID subunit 1, Transcription initiation factor TFIID subunit 12, Transcription initiation factor IIA subunit 2, ... (37 entities in total)
• Functional Keywords: tfiid, preinitiation complex, core promoter, transcription initiation, transcription
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 41
• Total formula weight: 2059760.01

Authors

Chen, X.,Wu, Z.,Hou, H.,Qi, Y.,Wang, X.,Li, J.,Xu, Y. (deposition date: 2021-03-24, release date: 2021-05-05, Last modification date: 2021-05-19)

Primary citation

Chen, X.,Qi, Y.,Wu, Z.,Wang, X.,Li, J.,Zhao, D.,Hou, H.,Li, Y.,Yu, Z.,Liu, W.,Wang, M.,Ren, Y.,Li, Z.,Yang, H.,Xu, Y.
Structural insights into preinitiation complex assembly on core promoters.
Science, 372:-, 2021

PubMed Abstract: Transcription factor IID (TFIID) recognizes core promoters and supports preinitiation complex (PIC) assembly for RNA polymerase II (Pol II)-mediated eukaryotic transcription. We determined the structures of human TFIID-based PIC in three stepwise assembly states and revealed two-track PIC assembly: stepwise promoter deposition to Pol II and extensive modular reorganization on track I (on TATA-TFIID-binding element promoters) versus direct promoter deposition on track II (on TATA-only and TATA-less promoters). The two tracks converge at an ~50-subunit holo PIC in identical conformation, whereby TFIID stabilizes PIC organization and supports loading of cyclin-dependent kinase (CDK)-activating kinase (CAK) onto Pol II and CAK-mediated phosphorylation of the Pol II carboxyl-terminal domain. Unexpectedly, TBP of TFIID similarly bends TATA box and TATA-less promoters in PIC. Our study provides structural visualization of stepwise PIC assembly on highly diversified promoters.

PubMed: 33795473
DOI: 10.1126/science.aba8490

Experimental method

ELECTRON MICROSCOPY (4.1 Å)