7EFT

Crystal structure of cell shape-determining protein MreC

7EFT の概要

• エントリーDOI: 10.2210/pdb7eft/pdb
• 分子名称: Cell shape protein MreC, CHLORIDE ION (3 entities in total)
• 機能のキーワード: scaffold protein, dimer, filament-like structure, structural protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 79207.38

構造登録者

Xu, Q.,Xiao, Q.J.,Sun, B. (登録日: 2021-03-23, 公開日: 2021-10-20, 最終更新日: 2024-10-23)

主引用文献

Xu, Q.,Sun, N.,Xiao, Q.,Huang, C.Y.,Xu, M.,Zhang, W.,Li, L.,Wang, Q.,Olieric, V.,Wang, W.,He, J.,Sun, B.
The crystal structure of MreC provides insights into polymer formation.
Febs Open Bio, 12:340-348, 2022

PubMed Abstract: MreC is a scaffold protein required for cell shape determination through interactions with proteins related to cell wall synthesis. Here, we determined the crystal structure of the major periplasmic part of MreC from Escherichia coli at 2.1 Å resolution. The periplasmic part of MreC contains a coiled-coil domain and two six-stranded barrel domains. The coiled-coil domain is essential for dimer formation, and the two monomers are prone to relative motion that is related to the small interface of β-barrel domains. In addition, MreC forms an antiparallel filament-like structure along the coiled-coil direction, which is different from the helical array structure in Pseudomonas aeruginosa. Our structure deepens our understanding of polymer formation of MreC.

PubMed: 34510818
DOI: 10.1002/2211-5463.13296

実験手法

X-RAY DIFFRACTION (2.1 Å)