7EEQ

Cyanophage Pam1 tail machine

Summary for 7EEQ

• Entry DOI: 10.2210/pdb7eeq/pdb
• EMDB information: 31080
• Descriptor: Needle head proteins, Tailspike head-binding domain (2 entities in total)
• Functional Keywords: needle head proteins and tailspike head-binding proteins, viral protein
• Biological source: unidentified; More
• Total number of polymer chains: 24
• Total formula weight: 485300.16

Authors

Zhang, J.T.,Jiang, Y.L.,Zhou, C.Z. (deposition date: 2021-03-19, release date: 2021-10-20, Last modification date: 2024-06-05)

Primary citation

Zhang, J.T.,Yang, F.,Du, K.,Li, W.F.,Chen, Y.,Jiang, Y.L.,Li, Q.,Zhou, C.Z.
Structure and assembly pattern of a freshwater short-tailed cyanophage Pam1.
Structure, 30:240-251.e4, 2022

PubMed Abstract: Despite previous structural analyses of bacteriophages, quite little is known about the structures and assembly patterns of cyanophages. Using cryo-EM combined with crystallography, we solve the near-atomic-resolution structure of a freshwater short-tailed cyanophage, Pam1, which comprises a 400-Å-long tail and an icosahedral capsid of 650 Å in diameter. The outer capsid surface is reinforced by trimeric cement proteins with a β-sandwich fold, which structurally resemble the distal motif of Pam1's tailspike, suggesting its potential role in host recognition. At the portal vertex, the dodecameric portal and connected adaptor, followed by a hexameric needle head, form a DNA ejection channel, which is sealed by a trimeric needle. Moreover, we identify a right-handed rifling pattern that might help DNA to revolve along the wall of the ejection channel. Our study reveals the precise assembly pattern of a cyanophage and lays the foundation to support its practical biotechnological and environmental applications.

PubMed: 34727518
DOI: 10.1016/j.str.2021.10.004

Experimental method

ELECTRON MICROSCOPY (3.96 Å)