7EEP

Cyanophage Pam1 portal-adaptor complex

7EEP の概要

• エントリーDOI: 10.2210/pdb7eep/pdb
• EMDBエントリー: 31079
• 分子名称: Pam1 portal proteins, Pam1 adaptor proteins (2 entities in total)
• 機能のキーワード: portal and adaptor proteins, virus
• 由来する生物種: unidentified; 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 1048619.30

構造登録者

Zhang, J.T.,Jiang, Y.L.,Zhou, C.Z. (登録日: 2021-03-19, 公開日: 2021-10-20, 最終更新日: 2024-06-05)

主引用文献

Zhang, J.T.,Yang, F.,Du, K.,Li, W.F.,Chen, Y.,Jiang, Y.L.,Li, Q.,Zhou, C.Z.
Structure and assembly pattern of a freshwater short-tailed cyanophage Pam1.
Structure, 30:240-251.e4, 2022

PubMed Abstract: Despite previous structural analyses of bacteriophages, quite little is known about the structures and assembly patterns of cyanophages. Using cryo-EM combined with crystallography, we solve the near-atomic-resolution structure of a freshwater short-tailed cyanophage, Pam1, which comprises a 400-Å-long tail and an icosahedral capsid of 650 Å in diameter. The outer capsid surface is reinforced by trimeric cement proteins with a β-sandwich fold, which structurally resemble the distal motif of Pam1's tailspike, suggesting its potential role in host recognition. At the portal vertex, the dodecameric portal and connected adaptor, followed by a hexameric needle head, form a DNA ejection channel, which is sealed by a trimeric needle. Moreover, we identify a right-handed rifling pattern that might help DNA to revolve along the wall of the ejection channel. Our study reveals the precise assembly pattern of a cyanophage and lays the foundation to support its practical biotechnological and environmental applications.

PubMed: 34727518
DOI: 10.1016/j.str.2021.10.004

実験手法

ELECTRON MICROSCOPY (3.75 Å)