7EDX

p53-bound TFIID-based core PIC on HDM2 promoter

7EDX の概要

• エントリーDOI: 10.2210/pdb7edx/pdb
• EMDBエントリー: 31075
• 分子名称: Transcription initiation factor TFIID subunit 1, Transcription initiation factor TFIID subunit 12, Transcription initiation factor IIA subunit 2, ... (35 entities in total)
• 機能のキーワード: tfiid, preinitiation complex, core promoter, transcription initiation, transcription
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 39
• 化学式量合計: 2026518.84

構造登録者

Chen, X.,Qi, Y.,Hou, H.,Wang, X.,Wu, Z.,Li, J.,Xu, Y. (登録日: 2021-03-17, 公開日: 2021-05-05, 最終更新日: 2024-10-23)

主引用文献

Chen, X.,Qi, Y.,Wu, Z.,Wang, X.,Li, J.,Zhao, D.,Hou, H.,Li, Y.,Yu, Z.,Liu, W.,Wang, M.,Ren, Y.,Li, Z.,Yang, H.,Xu, Y.
Structural insights into preinitiation complex assembly on core promoters.
Science, 372:-, 2021

PubMed Abstract: Transcription factor IID (TFIID) recognizes core promoters and supports preinitiation complex (PIC) assembly for RNA polymerase II (Pol II)-mediated eukaryotic transcription. We determined the structures of human TFIID-based PIC in three stepwise assembly states and revealed two-track PIC assembly: stepwise promoter deposition to Pol II and extensive modular reorganization on track I (on TATA-TFIID-binding element promoters) versus direct promoter deposition on track II (on TATA-only and TATA-less promoters). The two tracks converge at an ~50-subunit holo PIC in identical conformation, whereby TFIID stabilizes PIC organization and supports loading of cyclin-dependent kinase (CDK)-activating kinase (CAK) onto Pol II and CAK-mediated phosphorylation of the Pol II carboxyl-terminal domain. Unexpectedly, TBP of TFIID similarly bends TATA box and TATA-less promoters in PIC. Our study provides structural visualization of stepwise PIC assembly on highly diversified promoters.

PubMed: 33795473
DOI: 10.1126/science.aba8490

実験手法

ELECTRON MICROSCOPY (4.5 Å)