7ECV

The Csy-AcrIF14 complex

7ECV の概要

• エントリーDOI: 10.2210/pdb7ecv/pdb
• EMDBエントリー: 31058
• 分子名称: Type I-F CRISPR-associated endoribonuclease Cas6/Csy4, Type I-F CRISPR-associated protein Csy1, CRISPR type I-F/YPEST-associated protein Csy2, ... (6 entities in total)
• 機能のキーワード: inhibitor, complex, immune system, immune system-rna complex, immune system/rna
• 由来する生物種: Pseudomonas aeruginosa; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 380556.87

構造登録者

Zhang, L.X.,Feng, Y. (登録日: 2021-03-13, 公開日: 2021-11-17, 最終更新日: 2025-07-02)

主引用文献

Liu, X.,Zhang, L.,Xiu, Y.,Gao, T.,Huang, L.,Xie, Y.,Yang, L.,Wang, W.,Wang, P.,Zhang, Y.,Yang, M.,Feng, Y.
Insights into the dual functions of AcrIF14 during the inhibition of type I-F CRISPR-Cas surveillance complex.
Nucleic Acids Res., 49:10178-10191, 2021

PubMed Abstract: CRISPR-Cas systems are bacterial adaptive immune systems, and phages counteract these systems using many approaches such as producing anti-CRISPR (Acr) proteins. Here, we report the structures of both AcrIF14 and its complex with the crRNA-guided surveillance (Csy) complex. Our study demonstrates that apart from interacting with the Csy complex to block the hybridization of target DNA to the crRNA, AcrIF14 also endows the Csy complex with the ability to interact with non-sequence-specific dsDNA as AcrIF9 does. Further structural studies of the Csy-AcrIF14-dsDNA complex and biochemical studies uncover that the PAM recognition loop of the Cas8f subunit of the Csy complex and electropositive patches within the N-terminal domain of AcrIF14 are essential for the non-sequence-specific dsDNA binding to the Csy-AcrIF14 complex, which is different from the mechanism of AcrIF9. Our findings highlight the prevalence of Acr-induced non-specific DNA binding and shed light on future studies into the mechanisms of such Acr proteins.

PubMed: 34432044
DOI: 10.1093/nar/gkab738

実験手法

ELECTRON MICROSCOPY (3.43 Å)