7EBD

Bacterial STING in complex with c-di-GMP

7EBD の概要

• エントリーDOI: 10.2210/pdb7ebd/pdb
• 分子名称: TIR-like domain-containing protein, ACETATE ION, 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one), ... (4 entities in total)
• 機能のキーワード: second messenger, complex, cyclic dinucleotide, antiviral pathway, signaling protein
• 由来する生物種: Prevotella corporis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38070.75

構造登録者

Ko, T.-P.,Wang, Y.-C.,Yang, C.-S.,Hou, M.-H.,Chen, Y. (登録日: 2021-03-09, 公開日: 2021-09-15, 最終更新日: 2024-10-16)

主引用文献

Ko, T.-P.,Wang, Y.-C.,Yang, C.-S.,Hou, M.-H.,Chen, C.-J.,Chiu, Y.-F.,Chen, Y.
Crystal structure and functional implication of bacterial STING.
Nat Commun, 13:26-26, 2022

PubMed Abstract: Mammalian innate immune sensor STING (STimulator of INterferon Gene) was recently found to originate from bacteria. During phage infection, bacterial STING sense c-di-GMP generated by the CD-NTase (cGAS/DncV-like nucleotidyltransferase) encoded in the same operon and signal suicide commitment as a defense strategy that restricts phage propagation. However, the precise binding mode of c-di-GMP to bacterial STING and the specific recognition mechanism are still elusive. Here, we determine two complex crystal structures of bacterial STING/c-di-GMP, which provide a clear picture of how c-di-GMP is distinguished from other cyclic dinucleotides. The protein-protein interactions further reveal the driving force behind filament formation of bacterial STING. Finally, we group the bacterial STING into two classes based on the conserved motif in β-strand lid, which dictate their ligand specificity and oligomerization mechanism, and propose an evolution-based model that describes the transition from c-di-GMP-dependent signaling in bacteria to 2'3'-cGAMP-dependent signaling in eukaryotes.

PubMed: 35013136
DOI: 10.1038/s41467-021-26583-3

実験手法

X-RAY DIFFRACTION (2.25 Å)