7EB1

Solution NMR structure of the RRM domain of RNA binding protein RBM3 from homo sapiens

7EB1 の概要

• エントリーDOI: 10.2210/pdb7eb1/pdb
• 分子名称: RNA-binding protein 3 (1 entity in total)
• 機能のキーワード: rna recognition motif (rrm), rna binding motif 3 (rbm3), rna binding protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9286.25

構造登録者

Boral, S.,Roy, S.,Basak, A.J.,Maiti, S.,Lee, W.,De, S. (登録日: 2021-03-08, 公開日: 2021-12-08, 最終更新日: 2024-05-15)

主引用文献

Roy, S.,Boral, S.,Maiti, S.,Kushwaha, T.,Basak, A.J.,Lee, W.,Basak, A.,Gholap, S.L.,Inampudi, K.K.,De, S.
Structural and dynamic studies of the human RNA binding protein RBM3 reveals the molecular basis of its oligomerization and RNA recognition.
Febs J., 289:2847-2864, 2022

PubMed Abstract: Human RNA-binding motif 3 protein (RBM3) is a cold-shock protein which functions in various aspects of global protein synthesis, cell proliferation and apoptosis by interacting with the components of basal translational machinery. RBM3 plays important roles in tumour progression and cancer metastasis, and also has been shown to be involved in neuroprotection and endoplasmic reticulum stress response. Here, we have solved the solution NMR structure of the N-terminal 84 residue RNA recognition motif (RRM) of RBM3. The remaining residues are rich in RGG and YGG motifs and are disordered. The RRM domain adopts a βαββαβ topology, which is found in many RNA-binding proteins. NMR-monitored titration experiments and molecular dynamic simulations show that the beta-sheet and two loops form the RNA-binding interface. Hydrogen bond, pi-pi and pi-cation are the key interactions between the RNA and the RRM domain. NMR, size exclusion chromatography and chemical cross-linking experiments show that RBM3 forms oligomers in solution, which is favoured by decrease in temperature, thus, potentially linking it to its function as a cold-shock protein. Temperature-dependent NMR studies revealed that oligomerization of the RRM domain occurs via nonspecific interactions. Overall, this study provides the detailed structural analysis of RRM domain of RBM3, its interaction with RNA and the molecular basis of its temperature-dependent oligomerization.

PubMed: 34837346
DOI: 10.1111/febs.16301

実験手法

SOLUTION NMR