7EAG

Crystal structure of the RAGATH-18 k-turn

Summary for 7EAG

• Entry DOI: 10.2210/pdb7eag/pdb
• Descriptor: RNA (5'-R(*GP*UP*CP*UP*AP*UP*GP*AP*AP*GP*GP*CP*UP*GP*GP*AP*GP*AP*C)-3') (1 entity in total)
• Functional Keywords: rna, motif, kink-turn
• Biological source: Clostridium perfringens SM101
• Total number of polymer chains: 6
• Total formula weight: 36946.33

Authors

Huang, L.,Lilley, D.M.J. (deposition date: 2021-03-07, release date: 2021-06-02, Last modification date: 2023-11-29)

Primary citation

Huang, L.,Liao, X.,Li, M.,Wang, J.,Peng, X.,Wilson, T.J.,Lilley, D.M.J.
Structure and folding of four putative kink turns identified in structured RNA species in a test of structural prediction rules.
Nucleic Acids Res., 49:5916-5924, 2021

PubMed Abstract: k-Turns are widespread key architectural elements that occur in many classes of RNA molecules. We have shown previously that their folding properties (whether or not they fold into their tightly kinked structure on addition of metal ions) and conformation depend on their local sequence, and we have elucidated a series of rules for prediction of these properties from sequence. In this work, we have expanded the rules for prediction of folding properties, and then applied the full set to predict the folding and conformation of four probable k-turns we have identified amongst 224 structured RNA species found in bacterial intergenenic regions by the Breaker lab (1). We have analyzed the ion-dependence of folding of the four k-turns using fluorescence resonance energy transfer, and determined the conformation of two of them using X-ray crystallography. We find that the experimental data fully conform to both the predicted folding and conformational properties. We conclude that our folding rules are robust, and can be applied to new k-turns of unknown characteristics with confidence.

PubMed: 33978763
DOI: 10.1093/nar/gkab333

Experimental method

X-RAY DIFFRACTION (2.5 Å)