7E8L

The structure of Spodoptera litura chemosensory protein

Summary for 7E8L

• Entry DOI: 10.2210/pdb7e8l/pdb
• Descriptor: Putative chemosensory protein CSP8 (2 entities in total)
• Functional Keywords: chemosensory protein, alpha-helical protein, structural protein
• Biological source: Spodoptera litura (Asian cotton leafworm)
• Total number of polymer chains: 1
• Total formula weight: 13997.67

Authors

Xie, W.,Jia, Q.,Zeng, H.,Xiao, N.,Tang, J.,Gao, S.,Zhang, J. (deposition date: 2021-03-02, release date: 2022-03-02, Last modification date: 2024-10-23)

Primary citation

Jia, Q.,Zeng, H.,Zhang, J.,Gao, S.,Xiao, N.,Tang, J.,Dong, X.,Xie, W.
The Crystal Structure of the Spodoptera litura Chemosensory Protein CSP8.
Insects, 12:-, 2021

PubMed Abstract: F. is a generalist herbivore and one of the most important economic pests feeding on about 300 host plants in many Asian countries. Specific insect behaviors can be stimulated after recognizing chemicals in the external environment through conserved chemosensory proteins (CSPs) in chemoreceptive organs, which are critical components of the olfactory systems. To explore its structural basis for ligand-recognizing capability, we solved the 2.3 Å crystal structure of the apoprotein of CSP8 (SlCSP8). The SlCSP8 protein displays a conserved spherical shape with a negatively charged surface. Our binding assays showed that SlCSP8 bound several candidate ligands with differential affinities, with rhodojaponin III being the most tightly bound ligand. Our crystallographic and biochemical studies provide important insight into the molecular recognition mechanism of the sensory protein SlCSP8 and the CSP family in general, and they suggest that CSP8 is critical for insects to identify rhodojaponin III, which may aid in the CSP-based rational drug design in the future.

PubMed: 34357261
DOI: 10.3390/insects12070602

Experimental method

X-RAY DIFFRACTION (2.3 Å)