7E4E

NMR solution structures of DNA minidumbbell containing a N1-methyladenine

Summary for 7E4E

• Entry DOI: 10.2210/pdb7e4e/pdb
• Descriptor: DNA (5'-D(*TP*TP*TP*(MA7)P*TP*TP*TP*A)-3'), SODIUM ION (2 entities in total)
• Functional Keywords: dna minidumbbell, n1-methyladenine, methylation, non-b dna, hoogsteen base pair, dna
• Biological source: Staphylococcus aureus
• Total number of polymer chains: 1
• Total formula weight: 2559.59

Authors

Wan, L.,Guo, P.,Lam, S.L. (deposition date: 2021-02-11, release date: 2021-04-07, Last modification date: 2024-05-01)

Primary citation

Wan, L.,Lam, S.L.,Lee, H.K.,Guo, P.
Effects of Adenine Methylation on the Structure and Thermodynamic Stability of a DNA Minidumbbell.
Int J Mol Sci, 22:-, 2021

PubMed Abstract: DNA methylation is a prevalent regulatory modification in prokaryotes and eukaryotes. -methyladenine (mA) and -methyladenine (mA) have been found to be capable of altering DNA structures via disturbing Watson-Crick base pairing. However, little has been known about their influences on non-B DNA structures, which are associated with genetic instabilities. In this work, we investigated the effects of mA and mA on both the structure and thermodynamic stability of a newly reported DNA minidumbbell formed by two TTTA tetranucleotide repeats. As revealed by the results of nuclear magnetic resonance spectroscopic studies, both mA and mA favored the formation of a T·mA and T·mA Hoogsteen base pair, respectively. More intriguingly, the mA and mA modifications brought about stabilization and destabilization effects on the DNA minidumbbell, respectively. This work provides new biophysical insights into the effects of adenine methylation on the structure and thermodynamic stability of DNA.

PubMed: 33807305
DOI: 10.3390/ijms22073633

Experimental method

SOLUTION NMR