7E3L

Ultrapotent SARS-CoV-2 neutralizing antibodies with protective efficacy against newly emerged mutational variants

7E3L の概要

• エントリーDOI: 10.2210/pdb7e3l/pdb
• EMDBエントリー: 30982 30983
• 分子名称: Spike glycoprotein, 58G6 heavy chain, 58G6 light chain, ... (4 entities in total)
• 機能のキーワード: covid-19, spike glycoprotein, virus, viral protein, antibody
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 572788.28

構造登録者

Guo, H.,Li, T.,Liu, F.,Gao, Y.,Ji, X.,Yang, H. (登録日: 2021-02-09, 公開日: 2021-09-15, 最終更新日: 2025-06-25)

主引用文献

Li, T.,Han, X.,Gu, C.,Guo, H.,Zhang, H.,Wang, Y.,Hu, C.,Wang, K.,Liu, F.,Luo, F.,Zhang, Y.,Hu, J.,Wang, W.,Li, S.,Hao, Y.,Shen, M.,Huang, J.,Long, Y.,Song, S.,Wu, R.,Mu, S.,Chen, Q.,Gao, F.,Wang, J.,Long, S.,Li, L.,Wu, Y.,Gao, Y.,Xu, W.,Cai, X.,Qu, D.,Zhang, Z.,Zhang, H.,Li, N.,Gao, Q.,Zhang, G.,He, C.,Wang, W.,Ji, X.,Tang, N.,Yuan, Z.,Xie, Y.,Yang, H.,Zhang, B.,Huang, A.,Jin, A.
Potent SARS-CoV-2 neutralizing antibodies with protective efficacy against newly emerged mutational variants.
Nat Commun, 12:6304-6304, 2021

PubMed Abstract: Accumulating mutations in the SARS-CoV-2 Spike (S) protein can increase the possibility of immune escape, challenging the present COVID-19 prophylaxis and clinical interventions. Here, 3 receptor binding domain (RBD) specific monoclonal antibodies (mAbs), 58G6, 510A5 and 13G9, with high neutralizing potency blocking authentic SARS-CoV-2 virus display remarkable efficacy against authentic B.1.351 virus. Surprisingly, structural analysis has revealed that 58G6 and 13G9 both recognize the steric region S on the RBD, overlapping the E484K mutation presented in B.1.351. Also, 58G6 directly binds to another region S in the RBD. Significantly, 58G6 and 510A5 both demonstrate prophylactic efficacy against authentic SARS-CoV-2 and B.1.351 viruses in the transgenic mice expressing human ACE2 (hACE2), protecting weight loss and reducing virus loads. Together, we have evidenced 2 potent neutralizing Abs with unique mechanism targeting authentic SARS-CoV-2 mutants, which can be promising candidates to fulfill the urgent needs for the prolonged COVID-19 pandemic.

PubMed: 34728625
DOI: 10.1038/s41467-021-26539-7

実験手法

ELECTRON MICROSCOPY (3.6 Å)