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7E3E

Crystal structure of Trypanosoma brucei cathepsin B R91C/T223C mutant

Summary for 7E3E
Entry DOI10.2210/pdb7e3e/pdb
DescriptorCysteine peptidase C (CPC), 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
Functional Keywordsin cell crystal, hydrolase
Biological sourceTrypanosoma brucei brucei (strain 927/4 GUTat10.1)
Total number of polymer chains1
Total formula weight38218.62
Authors
Abe, S.,Pham, T.T.,Negishi, H.,Yamashita, K.,Hirata, K.,Ueno, T. (deposition date: 2021-02-08, release date: 2021-12-15, Last modification date: 2024-11-13)
Primary citationAbe, S.,Pham, T.T.,Negishi, H.,Yamashita, K.,Hirata, K.,Ueno, T.
Design of an In-Cell Protein Crystal for the Environmentally Responsive Construction of a Supramolecular Filament.
Angew.Chem.Int.Ed.Engl., 60:12341-12345, 2021
Cited by
PubMed Abstract: Protein assemblies can be designed for development of nano-bio materials. This has been achieved by modulating protein-protein interactions. However, fabrication of highly ordered protein assemblies remains challenging. Protein crystals, which have highly ordered arrangements of protein molecules, provide useful source matrices for synthesizing artificial protein assemblies. Here, we describe construction of a supramolecular filament structure by engineering covalent and non-covalent interactions in a protein crystal. Performing in-cell crystallization of Trypanosoma brucei cysteine protease cathepsin B (TbCatB), we achieved a precise arrangement of protein molecules while suppressing random aggregation due to disulfide bonds. We succeeded in synthesizing bundled filament from the crystals by autoxidation of cysteinyl thiols after the isolation of the crystals from living cells.
PubMed: 33759310
DOI: 10.1002/anie.202102039
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.3 Å)
Structure validation

237735

数据于2025-06-18公开中

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