7E1S
Crystal structure of dehydrogenase/isomerase FabX from Helicobacter pylori in complex with octanoyl-ACP
Summary for 7E1S
| Entry DOI | 10.2210/pdb7e1s/pdb |
| Descriptor | 2-nitropropane dioxygenase, Acyl carrier protein,Acyl carrier protein, FLAVIN MONONUCLEOTIDE, ... (6 entities in total) |
| Functional Keywords | unsaturated fatty acid biosynthesis, fmn, 4fe-4s cluster, flavoprotein |
| Biological source | Helicobacter pylori More |
| Total number of polymer chains | 4 |
| Total formula weight | 103054.72 |
| Authors | Zhou, J.S.,Zhang, L.,Zhang, L. (deposition date: 2021-02-03, release date: 2021-12-01, Last modification date: 2023-11-29) |
| Primary citation | Zhou, J.,Zhang, L.,Zeng, L.,Yu, L.,Duan, Y.,Shen, S.,Hu, J.,Zhang, P.,Song, W.,Ruan, X.,Jiang, J.,Zhang, Y.,Zhou, L.,Jia, J.,Hang, X.,Tian, C.,Lin, H.,Chen, H.Z.,Cronan, J.E.,Bi, H.,Zhang, L. Helicobacter pylori FabX contains a [4Fe-4S] cluster essential for unsaturated fatty acid synthesis. Nat Commun, 12:6932-6932, 2021 Cited by PubMed Abstract: Unsaturated fatty acids (UFAs) are essential for functional membrane phospholipids in most bacteria. The bifunctional dehydrogenase/isomerase FabX is an essential UFA biosynthesis enzyme in the widespread human pathogen Helicobacter pylori, a bacterium etiologically related to 95% of gastric cancers. Here, we present the crystal structures of FabX alone and in complexes with an octanoyl-acyl carrier protein (ACP) substrate or with holo-ACP. FabX belongs to the nitronate monooxygenase (NMO) flavoprotein family but contains an atypical [4Fe-4S] cluster absent in all other family members characterized to date. FabX binds ACP via its positively charged α7 helix that interacts with the negatively charged α2 and α3 helices of ACP. We demonstrate that the [4Fe-4S] cluster potentiates FMN oxidation during dehydrogenase catalysis, generating superoxide from an oxygen molecule that is locked in an oxyanion hole between the FMN and the active site residue His182. Both the [4Fe-4S] and FMN cofactors are essential for UFA synthesis, and the superoxide is subsequently excreted by H. pylori as a major resource of peroxide which may contribute to its pathogenic function in the corrosion of gastric mucosa. PubMed: 34836944DOI: 10.1038/s41467-021-27148-0 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.31 Å) |
Structure validation
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