7E1H

crystal structure of RD-BEF

7E1H の概要

• エントリーDOI: 10.2210/pdb7e1h/pdb
• 分子名称: DNA-binding response regulator, BERYLLIUM TRIFLUORIDE ION, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: response regulator, receiver domain, phosphorylation, signaling protein
• 由来する生物種: Vibrio parahaemolyticus
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 183756.50

構造登録者

Hong, S.,Zhang, X.,Zhang, P. (登録日: 2021-02-01, 公開日: 2022-02-09, 最終更新日: 2023-11-29)

主引用文献

Hong, S.,Guo, J.,Zhang, X.,Zhou, X.,Zhang, P.,Yu, F.
Structural basis of phosphorylation-induced activation of the response regulator VbrR.
Acta Biochim.Biophys.Sin., 2023

PubMed Abstract: Two-component systems typically consist of a paired histidine kinase and response regulator and couple environmental changes to adaptive responses. The response regulator VbrR from , a member of the OmpR/PhoB family, regulates virulence and antibiotic resistance genes. The activation mechanism of VbrR remains unclear. Here, we report the crystal structures of full-length VbrR in complex with DNA in the active conformation and the N-terminal receiver domain (RD) and the C-terminal DNA-binding domain (DBD) in both active and inactive conformations. Structural and biochemical analyses suggest that unphosphorylated VbrR adopts mainly as inactive dimers through the DBD at the autoinhibitory state. The RD undergoes a monomer-to-dimer transition upon phosphorylation, which further induces the transition of DBD from an autoinhibitory dimer to an active dimer and enables its binding with target DNA. Our study suggests a new model for phosphorylation-induced activation of response regulators and sheds light on the pathogenesis of . .

PubMed: 36647726
DOI: 10.3724/abbs.2022200

実験手法

X-RAY DIFFRACTION (2.805 Å)