7DZ8

State transition supercomplex PSI-LHCI-LHCII from the LhcbM1 lacking mutant of Chlamydomonas reinhardtii

7DZ8 の概要

• エントリーDOI: 10.2210/pdb7dz8/pdb
• EMDBエントリー: 30926
• 分子名称: Photosystem I P700 chlorophyll a apoprotein A1, Photosystem I reaction center subunit IX, Photosystem I reaction center subunit psaK, chloroplastic, ... (37 entities in total)
• 機能のキーワード: supercomplex, state transition, green alga, chlamydomonas reinhardtii, photosynthesis
• 由来する生物種: Chlamydomonas reinhardtii (Chlamydomonas smithii); 詳細
• タンパク質・核酸の鎖数: 29
• 化学式量合計: 1132208.42

構造登録者

Pan, X.W.,Li, A.J.,Liu, Z.F.,Li, M. (登録日: 2021-01-23, 公開日: 2021-06-30, 最終更新日: 2024-10-09)

主引用文献

Pan, X.,Tokutsu, R.,Li, A.,Takizawa, K.,Song, C.,Murata, K.,Yamasaki, T.,Liu, Z.,Minagawa, J.,Li, M.
Structural basis of LhcbM5-mediated state transitions in green algae.
Nat.Plants, 7:1119-1131, 2021

PubMed Abstract: In green algae and plants, state transitions serve as a short-term light-acclimation process in the regulation of the light-harvesting capacity of photosystems I and II (PSI and PSII, respectively). During the process, a portion of light-harvesting complex II (LHCII) is phosphorylated, dissociated from PSII and binds with PSI to form the supercomplex PSI-LHCI-LHCII. Here, we report high-resolution structures of PSI-LHCI-LHCII from Chlamydomonas reinhardtii, revealing the mechanism of assembly between the PSI-LHCI complex and two phosphorylated LHCII trimers containing all four types of LhcbM protein. Two specific LhcbM isoforms, namely LhcbM1 and LhcbM5, directly interact with the PSI core through their phosphorylated amino terminal regions. Furthermore, biochemical and functional studies on mutant strains lacking either LhcbM1 or LhcbM5 indicate that only LhcbM5 is indispensable in supercomplex formation. The results unravel the specific interactions and potential excitation energy transfer routes between green algal PSI and two phosphorylated LHCIIs.

PubMed: 34239095
DOI: 10.1038/s41477-021-00960-8

実験手法

ELECTRON MICROSCOPY (3.16 Å)