7DWS

The structure of T4 Lysozyme I3C/C54T/R125C/E128C complex with Zinc ions

Summary for 7DWS

• Entry DOI: 10.2210/pdb7dws/pdb
• Descriptor: Endolysin, ZINC ION (3 entities in total)
• Functional Keywords: muramidase, enzyme, mutant, metal ion, antimicrobial protein, hydrolase
• Biological source: Enterobacteria phage T4 (Bacteriophage T4)
• Total number of polymer chains: 3
• Total formula weight: 58750.58

Authors

Chen, X.,Chen, S. (deposition date: 2021-01-17, release date: 2021-06-02, Last modification date: 2024-10-30)

Primary citation

Chen, X.,Tan, B.,Wang, S.,Tang, R.,Bao, Z.,Chen, G.,Chen, S.,Tang, W.,Wang, Z.,Long, C.,Lu, W.W.,Yang, D.,Bian, L.,Peng, S.
Rationally designed protein cross-linked hydrogel for bone regeneration via synergistic release of magnesium and zinc ions.
Biomaterials, 274:120895-120895, 2021

PubMed Abstract: The development of recombinant protein cross-linked injectable hydrogels with good mechanical strength and effective drug loading capacity for bone regeneration is extremely attractive and rarely reported. Here, we report the fabrication of a smart hydrogel delivery system by incorporating a rationally designed T4 lysozyme mutant (T4M) to mediate the localized delivery and synergistic release of Mg and Zn for bone repair. Apart from its intrinsic antibacterial properties, T4M bears abundant free amine groups on its surface to function as effective covalent crosslinkers to strengthen the hydrogel network as well as exhibits specific binding affinity to multivalent cations such as Zn. Moreover, the integrin receptor-binding Arg-Gly-Asp (RGD) sequence was introduced onto the C-terminus of T4 lysozyme to improve its cellular affinity and further facilitate rapid tissue regeneration. The final composite hydrogel displays excellent injectability, improved mechanical properties, antibacterial activity, and unique bioactivities. The effective loading of Mg/Zn in the hydrogels could mediate the sequential and sustained release of Mg and Zn, thereby resulting in synergistic enhancement on bone regeneration through modulation of the MAPK signaling pathway. We believe that the strategy proposed in this paper opens up a new route for developing protein cross-linked smart delivery systems for tissue regeneration.

PubMed: 34020269
DOI: 10.1016/j.biomaterials.2021.120895

Experimental method

X-RAY DIFFRACTION (2.8 Å)