7DTL

Crystal structure of PSK, an antimicrobial peptide from Chrysomya megacephala

7DTL の概要

• エントリーDOI: 10.2210/pdb7dtl/pdb
• 分子名称: PSK (2 entities in total)
• 機能のキーワード: antimicrobial peptide, mitochondrial atpase inhibitor, psk, antimicrobial protein
• 由来する生物種: Chrysomya megacephala
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 20823.05

構造登録者

Xiao, C.,Xiao, Z.,Wang, S.,Liu, W. (登録日: 2021-01-05, 公開日: 2021-07-14, 最終更新日: 2024-03-27)

主引用文献

Xiao, C.,Xiao, Z.,Hu, C.,Lu, J.,Cui, L.,Zhang, Y.,Dai, Y.,Zhang, Q.,Wang, S.,Liu, W.
Crystal and solution structures of a novel antimicrobial peptide from Chrysomya megacephala.
Acta Crystallogr D Struct Biol, 77:894-903, 2021

PubMed Abstract: Antimicrobial peptides (AMPs) are small amphipathic peptides that exhibit bactericidal activity against a wide range of pathogenic microorganisms and are considered to be potential substitutes for antibiotics effective against microbial infection. PSK, an 84-amino-acid AMP recently isolated from Chrysomya megacephala larvae, probably belongs to the mitochondrial ATPase inhibitor family according to its sequence. No member of this family from an insect has been structurally characterized to date. In this study, the crystal structure of full-length PSK determined by molecular replacement using an ab initio modeled ensemble as a search model and a solution structure obtained from small-angle X-ray scattering (SAXS) measurements are reported. The crystal structure reveals a distinct fold compared with those of homologous peptides, in that PSK comprises two antiparallel α-helices rather than a single long helix, which is in good agreement with the SAXS-based ab initio model. However, the peptide exists as a monomer in solution, even though a stable dimer was observed in the crystal structure. This apparent contradiction may reflect different oligomerization states that may be implicated in its bioactivity. The data presented here have established a solid basis for further mechanistic studies of this novel insect AMP.

PubMed: 34196616
DOI: 10.1107/S2059798321004629

実験手法

X-RAY DIFFRACTION (1.8 Å)