7DRG

Pseudomonas aeruginosa T6SS protein PA0821

Summary for 7DRG

• Entry DOI: 10.2210/pdb7drg/pdb
• Descriptor: PA0821 (2 entities in total)
• Functional Keywords: t6ss, antitoxin
• Biological source: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
• Total number of polymer chains: 2
• Total formula weight: 82550.83

Authors

She, Z. (deposition date: 2020-12-28, release date: 2021-06-16, Last modification date: 2024-11-13)

Primary citation

Wang, S.,Geng, Z.,Zhang, H.,She, Z.,Dong, Y.
The Pseudomonas aeruginosa PAAR2 cluster encodes a putative VRR-NUC domain-containing effector.
Febs J., 288:5755-5767, 2021

PubMed Abstract: The bacterial type VI secretion system (T6SS) secretes many toxic effectors to gain advantage in inter-bacterial competition and for eukaryotic host infection. The cognate immunity proteins of these effectors protect bacteria from the virulence of their own effectors. The T6SS injects its inner-needle Hcp tube, the sharpening tip complex -consisting of VgrG and proline-alanine-alanine-arginine repeats (PAAR) proteins- and toxic effectors into neighboring cells. Its functions are largely determined by the activities of its delivered effectors. Five PAAR proteins were found in the Pseudomonas aeruginosa PAO1 genome with three of them shown to facilitate the delivery of various effectors. Here, we report a putative virus-type replication-repair nuclease domain-containing effector TseV encoded by the least investigated P. aeruginosa PAAR2 cluster. The crystal structure of its putative cognate effector TsiV is presented at 1.6 Å resolution. Through structure and sequence comparisons, we propose TseV-TsiV to be a putative novel effector-immunity (E-I) pair and we discuss the roles of other PAAR2 cluster encoded proteins.

PubMed: 33838074
DOI: 10.1111/febs.15870

Experimental method

X-RAY DIFFRACTION (1.6 Å)