7DOH

Crystal Structure of GD-26 Fab in Complex with TD Peptide from Haloarcula Marismortui Bacteriorhodopsin I

7DOH の概要

• エントリーDOI: 10.2210/pdb7doh/pdb
• 分子名称: GLY-THR-GLY-ALA-THR-PRO-ALA-ASP-ASP, GD-26 Fab L-chain, GD-26 Fab H-chain, ... (4 entities in total)
• 機能のキーワード: antibody, immune system
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 49596.25

構造登録者

Lee, C.C.,Pao, P.J.,Wang, A.H.J. (登録日: 2020-12-14, 公開日: 2021-10-27, 最終更新日: 2024-11-20)

主引用文献

Pao, P.J.,Hsu, M.F.,Chiang, M.H.,Chen, C.T.,Lee, C.C.,Wang, A.H.
Structural basis of an epitope tagging system derived from Haloarcula marismortui bacteriorhodopsin I D94N and its monoclonal antibody GD-26.
Febs J., 289:730-747, 2022

PubMed Abstract: Specific antibody interactions with short peptides have made epitope tagging systems a vital tool employed in virtually all fields of biological research. Here, we present a novel epitope tagging system comprised of a monoclonal antibody named GD-26, which recognises the TD peptide (GTGATPADD) derived from Haloarcula marismortui bacteriorhodopsin I (HmBRI) D94N mutant. The crystal structure of the antigen-binding fragment (Fab) of GD-26 complexed with the TD peptide was determined to a resolution of 1.45 Å. The TD peptide was found to adopt a 3 helix conformation within the binding cleft, providing a characteristic peptide structure for recognition by GD-26 Fab. Based on the structure information, polar and nonpolar forces collectively contribute to the strong binding. Attempts to engineer the TD peptide show that the proline residue is crucial for the formation of the 3 helix in order to fit into the binding cleft. Isothermal calorimetry (ITC) reported a dissociation constant K of 12 ± 2.8 nm, indicating a strong interaction between the TD peptide and GD-26 Fab. High specificity of GD-26 IgG to the TD peptide was demonstrated by western blotting, ELISA and immunofluorescence as only TD-tagged proteins were detected, suggesting the effectiveness of the GD-26/TD peptide tagging system. In addition to already-existing epitope tags such as the FLAG tag and the ALFA tag adopting either extended or α-helix conformations, the unique 3 helix conformation of the TD peptide together with the corresponding monoclonal antibody GD-26 offers a novel tagging option for research.

PubMed: 34499806
DOI: 10.1111/febs.16184

実験手法

X-RAY DIFFRACTION (1.45 Å)