7DH6

Crystal structure of PLRG1

Summary for 7DH6

• Entry DOI: 10.2210/pdb7dh6/pdb
• Descriptor: Pleiotropic regulator 1, NICKEL (II) ION, SULFATE ION, ... (5 entities in total)
• Functional Keywords: plrg1 offers an interesting link between the control of pre-mrna splicing and dna metabolism., splicing
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 4
• Total formula weight: 167659.04

Authors

Wang, X.,Xu, C. (deposition date: 2020-11-13, release date: 2020-12-02, Last modification date: 2023-11-29)

Primary citation

Wang, X.,Li, Y.,Dai, H.,Xu, C.
Crystal structure of the WD40 domain of human PLRG1.
Biochem.Biophys.Res.Commun., 534:474-477, 2021

PubMed Abstract: PLRG1 is a evolutionarily conserved protein in spliceosome and plays an important role in maintaining the integral part of the splicoeosme and its proper splicing. Here we solved the high resolution crystal structure of the WD40 domain of human PLRG1 by crystallography and compared our crystal structure with the cryo-EM structure of PLRG1 bound with other splicing factors. We found that two loops of the WD40 domain become resolved upon binding to the proteins within the spliceosome. Thus our work characterize the dynamic property of PLRG1 during the spliceosome assembly by presenting its apo structure.

PubMed: 33239170
DOI: 10.1016/j.bbrc.2020.11.057

Experimental method

X-RAY DIFFRACTION (2.584 Å)