7DH6

Crystal structure of PLRG1

7DH6 の概要

• エントリーDOI: 10.2210/pdb7dh6/pdb
• 分子名称: Pleiotropic regulator 1, NICKEL (II) ION, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: plrg1 offers an interesting link between the control of pre-mrna splicing and dna metabolism., splicing
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 167659.04

構造登録者

Wang, X.,Xu, C. (登録日: 2020-11-13, 公開日: 2020-12-02, 最終更新日: 2023-11-29)

主引用文献

Wang, X.,Li, Y.,Dai, H.,Xu, C.
Crystal structure of the WD40 domain of human PLRG1.
Biochem.Biophys.Res.Commun., 534:474-477, 2021

PubMed Abstract: PLRG1 is a evolutionarily conserved protein in spliceosome and plays an important role in maintaining the integral part of the splicoeosme and its proper splicing. Here we solved the high resolution crystal structure of the WD40 domain of human PLRG1 by crystallography and compared our crystal structure with the cryo-EM structure of PLRG1 bound with other splicing factors. We found that two loops of the WD40 domain become resolved upon binding to the proteins within the spliceosome. Thus our work characterize the dynamic property of PLRG1 during the spliceosome assembly by presenting its apo structure.

PubMed: 33239170
DOI: 10.1016/j.bbrc.2020.11.057

実験手法

X-RAY DIFFRACTION (2.584 Å)