7D8O

Crystal structure of E. coli ToxIN type III toxin-antitoxin complex

7D8O の概要

• エントリーDOI: 10.2210/pdb7d8o/pdb
• 分子名称: Type III toxin-antitoxin system ToxN/AbiQ family toxin, Antitoxin RNA (3 entities in total)
• 機能のキーワード: toxin-antitoxin complex, toxin, endoribonuclease, rna pseudoknot, rna-protein complex, toxin-rna complex, toxin/rna
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 201695.89

構造登録者

Manikandan, P.,Rothweiler, U.,Singh, M. (登録日: 2020-10-08, 公開日: 2022-01-05, 最終更新日: 2023-11-29)

主引用文献

Manikandan, P.,Sandhya, S.,Nadig, K.,Paul, S.,Srinivasan, N.,Rothweiler, U.,Singh, M.
Identification, functional characterization, assembly and structure of ToxIN type III toxin-antitoxin complex from E. coli.
Nucleic Acids Res., 50:1687-1700, 2022

PubMed Abstract: Toxin-antitoxin (TA) systems are proposed to play crucial roles in bacterial growth under stress conditions such as phage infection. The type III TA systems consist of a protein toxin whose activity is inhibited by a noncoding RNA antitoxin. The toxin is an endoribonuclease, while the antitoxin consists of multiple repeats of RNA. The toxin assembles with the individual antitoxin repeats into a cyclic complex in which the antitoxin forms a pseudoknot structure. While structure and functions of some type III TA systems are characterized, the complex assembly process is not well understood. Using bioinformatics analysis, we have identified type III TA systems belonging to the ToxIN family across different Escherichia coli strains and found them to be clustered into at least five distinct clusters. Furthermore, we report a 2.097 Å resolution crystal structure of the first E. coli ToxIN complex that revealed the overall assembly of the protein-RNA complex. Isothermal titration calorimetry experiments showed that toxin forms a high-affinity complex with antitoxin RNA resulting from two independent (5' and 3' sides of RNA) RNA binding sites on the protein. These results further our understanding of the assembly of type III TA complexes in bacteria.

PubMed: 35018473
DOI: 10.1093/nar/gkab1264

実験手法

X-RAY DIFFRACTION (2.097 Å)