7D8K

Solution structure of the methyl-CpG binding domain of MBD6 from Arabidopsis thaliana

7D8K の概要

• エントリーDOI: 10.2210/pdb7d8k/pdb
• 分子名称: Methyl-CpG-binding domain-containing protein 6 (1 entity in total)
• 機能のキーワード: methyl-cpg binding, gene repressor, dna binding protein
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7770.58

構造登録者

Mahana, Y.,Ohki, I.,Walinda, E.,Morimoto, D.,Sugase, K.,Shirakawa, M. (登録日: 2020-10-08, 公開日: 2021-10-20, 最終更新日: 2024-05-15)

主引用文献

Mahana, Y.,Ohki, I.,Walinda, E.,Morimoto, D.,Sugase, K.,Shirakawa, M.
Structural Insights into Methylated DNA Recognition by the Methyl-CpG Binding Domain of MBD6 from Arabidopsis thaliana .
Acs Omega, 7:3212-3221, 2022

PubMed Abstract: Cytosine methylation is an epigenetic modification essential for formation of mature heterochromatin, gene silencing, and genomic stability. In plants, methylation occurs not only at cytosine bases in CpG but also in CpHpG and CpHpH contexts, where H denotes A, T, or C. Methyl-CpG binding domain (MBD) proteins, which recognize symmetrical methyl-CpG dinucleotides and act as gene repressors in mammalian cells, are also present in plant cells, although their structural and functional properties still remain poorly understood. To fill this gap, in this study, we determined the solution structure of the MBD domain of the MBD6 protein from and investigated its binding properties to methylated DNA by binding assays and an in-depth NMR spectroscopic analysis. The AtMBD6 MBD domain folds into a canonical MBD structure in line with its binding specificity toward methyl-CpG and possesses a DNA binding interface similar to mammalian MBD domains. Intriguingly, however, the binding affinity of the AtMBD6 MBD domain toward methyl-CpG-containing DNA was found to be much lower than that of known mammalian MBD domains. The main difference arises from the absence of positively charged residues in AtMBD6 that supposedly interact with the DNA backbone as seen in mammalian MBD/methyl-CpG-containing DNA complexes. Taken together, we have established a structural basis for methyl-CpG recognition by AtMBD6 to develop a deeper understanding how MBD proteins work as mediators of epigenetic signals in plant cells.

PubMed: 35128234
DOI: 10.1021/acsomega.1c04917

実験手法

SOLUTION NMR