7D8H

CRTC1 pSer64 peptide in complex with 14-3-3 zeta

7D8H の概要

• エントリーDOI: 10.2210/pdb7d8h/pdb
• 分子名称: 14-3-3 protein zeta/delta, CRTC1 pSer64 peptide (3 entities in total)
• 機能のキーワード: 14-3-3, crtc, complex, protein binding
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 31239.72

構造登録者

Chen, H.,Zhang, H.,Xiang, S. (登録日: 2020-10-08, 公開日: 2021-02-24, 最終更新日: 2024-11-20)

主引用文献

Chen, H.,Zhang, H.,Chen, P.,Xiang, S.
Structural Insights into the Interaction Between CRTCs and 14-3-3.
J.Mol.Biol., 433:166874-166874, 2021

PubMed Abstract: The CREB-Regulated Transcriptional Coactivators (CRTCs) regulate the transcription of CREB target genes and have important functions in many biological processes. At the basal state, they are phosphorylated at multiple residues, which promotes their association with 14-3-3 that sequesters them in the cytoplasm. Upon dephosphorylation, they translocate into the nuclei and associate with CREB to activate the target gene transcription. Although three conserved serine residues in CRTCs have been implicated in their phosphorylation regulation, whether and how they mediate interactions with 14-3-3 is unclear. Here, we provide direct evidence that these residues and flanking regions interact with 14-3-3 and the structural basis of the interaction. Our study also identified a novel salt bridge in CRTC1 with an important function in binding 14-3-3, expanding the understanding of the interaction between 14-3-3 and its ligands.

PubMed: 33556406
DOI: 10.1016/j.jmb.2021.166874

実験手法

X-RAY DIFFRACTION (2.42 Å)