7D7V

Crystal Structure of the Domain1 of NAD+ Riboswitch with nicotinamide adenine dinucleotide (NAD+) and U1A protein

7D7V の概要

• エントリーDOI: 10.2210/pdb7d7v/pdb
• 分子名称: 17delU1A (58-MER), U1 small nuclear ribonucleoprotein A, GUANOSINE-5'-TRIPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: riboswitch, rna structure, rna folding, rna-ligand interactions, rna crystallography, rna
• 由来する生物種: Acidobacterium capsulatum ATCC 51196; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 30333.37

構造登録者

Chen, H.,Ren, A.M. (登録日: 2020-10-06, 公開日: 2020-11-25, 最終更新日: 2024-03-27)

主引用文献

Chen, H.,Egger, M.,Xu, X.,Flemmich, L.,Krasheninina, O.,Sun, A.,Micura, R.,Ren, A.
Structural distinctions between NAD+ riboswitch domains 1 and 2 determine differential folding and ligand binding.
Nucleic Acids Res., 48:12394-12406, 2020

PubMed Abstract: Riboswitches are important gene regulatory elements frequently encountered in bacterial mRNAs. The recently discovered nadA riboswitch contains two similar, tandemly arrayed aptamer domains, with the first domain possessing high affinity for nicotinamide adenine dinucleotide (NAD+). The second domain which comprises the ribosomal binding site in a putative regulatory helix, however, has withdrawn from detection of ligand-induced structural modulation thus far, and therefore, the identity of the cognate ligand and the regulation mechanism have remained unclear. Here, we report crystal structures of both riboswitch domains, each bound to NAD+. Furthermore, we demonstrate that ligand binding to domain 2 requires significantly higher concentrations of NAD+ (or ADP retaining analogs) compared to domain 1. Using a fluorescence spectroscopic approach, we further shed light on the structural features which are responsible for the different ligand affinities, and describe the Mg2+-dependent, distinct folding and pre-organization of their binding pockets. Finally, we speculate about possible scenarios for nadA RNA gene regulation as a putative two-concentration sensor module for a time-controlled signal that is primed and stalled by the gene regulation machinery at low ligand concentrations (domain 1), and finally triggers repression of translation as soon as high ligand concentrations are reached in the cell (domain 2).

PubMed: 33170270
DOI: 10.1093/nar/gkaa1029

実験手法

X-RAY DIFFRACTION (2.8 Å)