7D79

The structure of DcsB complex with its substrate analogue

7D79 の概要

• エントリーDOI: 10.2210/pdb7d79/pdb
• 分子名称: DltD domain-containing protein, CHLORIDE ION, 1,2-ETHANEDIOL, ... (8 entities in total)
• 機能のキーワード: thioesterase, complex, hydrolase
• 由来する生物種: Beauveria bassiana (strain ARSEF 2860) (White muscardine disease fungus)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 141839.02

構造登録者

Tang, Y.,Zhou, J.H.,Wang, G.Q. (登録日: 2020-10-03, 公開日: 2021-01-27, 最終更新日: 2024-03-27)

主引用文献

Gao, D.W.,Jamieson, C.S.,Wang, G.,Yan, Y.,Zhou, J.,Houk, K.N.,Tang, Y.
A Polyketide Cyclase That Forms Medium-Ring Lactones.
J.Am.Chem.Soc., 143:80-84, 2021

PubMed Abstract: Medium-ring lactones are synthetically challenging due to unfavorable energetics involved in cyclization. We have discovered a thioesterase enzyme DcsB, from the decarestrictine C1 () biosynthetic pathway, that efficiently performs medium-ring lactonizations. DcsB shows broad substrate promiscuity toward linear substrates that vary in lengths and substituents, and is a potential biocatalyst for lactonization. X-ray crystal structure and computational analyses provide insights into the molecular basis of catalysis.

PubMed: 33351624
DOI: 10.1021/jacs.0c11226

実験手法

X-RAY DIFFRACTION (2.10411425302 Å)