7D5K

CryoEM structure of cotton cellulose synthase isoform 7

7D5K の概要

• エントリーDOI: 10.2210/pdb7d5k/pdb
• EMDBエントリー: 30583
• 関連するBIRD辞書のPRD_ID: PRD_900005
• 分子名称: Cellulose synthase, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose (2 entities in total)
• 機能のキーワード: cellulose synthase, cotton, membrane protein
• 由来する生物種: Gossypium hirsutum (Upland cotton)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 356442.91

構造登録者

Guan, Z.Y.,Xue, Y.,Yin, P.,Zhang, X.L. (登録日: 2020-09-26, 公開日: 2021-07-28, 最終更新日: 2024-05-29)

主引用文献

Zhang, X.,Xue, Y.,Guan, Z.,Zhou, C.,Nie, Y.,Men, S.,Wang, Q.,Shen, C.,Zhang, D.,Jin, S.,Tu, L.,Yin, P.,Zhang, X.
Structural insights into homotrimeric assembly of cellulose synthase CesA7 from Gossypium hirsutum.
Plant Biotechnol J, 19:1579-1587, 2021

PubMed Abstract: Cellulose is one of the most abundant organic polymers in nature. It contains multiple β-1,4-glucan chains synthesized by cellulose synthases (CesAs) on the plasma membrane of higher plants. CesA subunits assemble into a pseudo-sixfold symmetric cellulose synthase complex (CSC), known as a 'rosette complex'. The structure of CesA remains enigmatic. Here, we report the cryo-EM structure of the homotrimeric CesA7 from Gossypium hirsutum at 3.5-angstrom resolution. The GhCesA7 homotrimer shows a C3 symmetrical assembly. Each protomer contains seven transmembrane helices (TMs) which form a channel potentially facilitating the release of newly synthesized glucans. The cytoplasmic glycosyltransferase domain (GT domain) of GhCesA7 protrudes from the membrane, and its catalytic pocket is directed towards the TM pore. The homotrimer GhCesA7 is stabilized by the transmembrane helix 7 (TM7) and the plant-conserved region (PCR) domains. It represents the building block of CSCs and facilitates microfibril formation. This structure provides insight into how eukaryotic cellulose synthase assembles and provides a mechanistic basis for the improvement of cotton fibre quality in the future.

PubMed: 33638282
DOI: 10.1111/pbi.13571

実験手法

ELECTRON MICROSCOPY (3.5 Å)